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Literature summary extracted from

  • Tomiya, N.; Narang, S.; Park, J.; Abdul-Rahman, B.; Choi, O.; Singh, S.; Hiratake, J.; Sakata, S.; Betenbaugh, M.J.; Palter, K.B.; Lee, Y.C.
    Purification, characterization, and cloning of a spodoptera frugiperda SF9 beta-N-acetylhexosaminidase that hydrolyzes terminal N-acetylglucosamine on N-glycan core (2006), J. Biol. Chem., 281, 19545-19560.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 2-acetamido-1,2-dideoxynojirimycin
-
 Spodoptera frugiperda
3.2.1.52 N-acetyl-D-glucosamine
-
 Spodoptera frugiperda

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.51
-
 4-nitrophenyl beta-N-acetyl-D-glucosaminide
-
 Spodoptera frugiperda

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 61000
-
 SDS-PAGE of de-N-glycosylated Sfhex Spodoptera frugiperda
3.2.1.52 67000
-
 SDS-PAGE of purified Sfhex Spodoptera frugiperda
3.2.1.52 110000
-
 gel filtration of Sfhex Spodoptera frugiperda

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Spodoptera frugiperda Q3LS76
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 SDS-PAGE and affinity chromatography Spodoptera frugiperda

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 SF-9 cell cell culture Spodoptera frugiperda
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 0.003
-
 GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside, desalted enzyme at pH 5.5 and 37°C Spodoptera frugiperda
3.2.1.52 0.035
-
 GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside, desalted enzyme at pH 5.5 and 37°C Spodoptera frugiperda

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-galactopyranoside + H2O
-
 Spodoptera frugiperda 4-methylumbelliferol + N-acetyl-beta-D-galactosamine
-
 ?
3.2.1.52 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
 Spodoptera frugiperda 4-methylumbelliferol + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 4-nitrophenyl beta-N-acetyl-D-glucosaminide + H2O
-
 Spodoptera frugiperda 4-methylumbelliferone + beta-N-acetyl-D-glucosamine
-
 ?
3.2.1.52 GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside + H2O
-
 Spodoptera frugiperda GlcNAcbeta(1-2)Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside + H2O
-
 Spodoptera frugiperda Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside + GlcNAcbeta(1-2)Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 GlcNAcbeta(1-2)Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside + H2O
-
 Spodoptera frugiperda Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside
-
 ?
3.2.1.52 GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside + H2O
-
 Spodoptera frugiperda ?
-
 ?
3.2.1.52 Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside + H2O
-
 Spodoptera frugiperda Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside
-
 ?
3.2.1.52 Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside + H2O
-
 Spodoptera frugiperda Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside
-
 ?
3.2.1.52 p-nitrophenyl-N-acetyl-glucosamine + H2O
-
 Spodoptera frugiperda p-nitrophenol + N-acetyl-glucosamine
-
 ?
3.2.1.52 [GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside + H2O
-
 Spodoptera frugiperda Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside + N-acetyl-D-glucosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 homodimer 2 * 61000, N-deglycosylated Sfhex, 2 * 67000, SDS-PAGE Spodoptera frugiperda

Synonyms

EC Number Synonyms Comment Organism
3.2.1.52 Beta-N-acetylhexosaminidase
-
 Spodoptera frugiperda
3.2.1.52 Sfhex
-
 Spodoptera frugiperda

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.52 0.0005
-
 GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside product MGn-2-aminopyridine Spodoptera frugiperda
3.2.1.52 0.0007
-
 GlcNAcbeta(1-2)Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside
-
 Spodoptera frugiperda
3.2.1.52 0.0012
-
 Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside
-
 Spodoptera frugiperda
3.2.1.52 0.0016
-
 Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside
-
 Spodoptera frugiperda
3.2.1.52 0.0025
-
 GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside product GnM-2-aminopyridine Spodoptera frugiperda

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 5.5
-
-
 Spodoptera frugiperda

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.52 0.00022
-
 2-acetamido-1,2-dideoxynojirimycin
-
 Spodoptera frugiperda
3.2.1.52 2
-
 N-acetyl-D-glucosamine
-
 Spodoptera frugiperda