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Literature summary extracted from

  • Gardiner, D.L.; Trenholme, K.R.; Skinner-Adams, T.S.; Stack, C.M.; Dalton, J.P.
    Overexpression of leucyl aminopeptidase in Plasmodium falciparum parasites. Target for the antimalarial activity of bestatin (2006), J. Biol. Chem., 281, 1741-1745.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.11.1 pharmacology the enzyme is a target for the antimalarial activity of inhibitor bestatin Plasmodium falciparum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.1 DNA and amino acid sequence determination and analysis, overexpression of the full-length gene in parasite cytosol, transgenic parasites are more resistant to bestatin Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.1 bestatin
-
 Plasmodium falciparum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.1 cytosol
-
 Plasmodium falciparum 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.1 65000
-
 x * 65000, recombinant enzyme, SDS-PAGE, x * 67831, sequence calculation Plasmodium falciparum
3.4.11.1 67831
-
 x * 65000, recombinant enzyme, SDS-PAGE, x * 67831, sequence calculation Plasmodium falciparum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.11.1 additional information Plasmodium falciparum the enzyme is important in the generation and regulation of free amino acids that are used in protein anabolism and for maintaining osmotic stability within the infected erythrocyte, enzyme inhibition, e.g. by bestatin, blocks intraerythrocytic development of malaria parasites ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Plasmodium falciparum
-
 clone D10 of isolate FC27
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.1 alanyl-7-amido-4-methylcoumarin + H2O
-
 Plasmodium falciparum alanine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 L-leucyl-7-amido-4-methylcoumarin + H2O
-
 Plasmodium falciparum L-leucine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 additional information the enzyme is important in the generation and regulation of free amino acids that are used in protein anabolism and for maintaining osmotic stability within the infected erythrocyte, enzyme inhibition, e.g. by bestatin, blocks intraerythrocytic development of malaria parasites Plasmodium falciparum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.1 ? x * 65000, recombinant enzyme, SDS-PAGE, x * 67831, sequence calculation Plasmodium falciparum

Synonyms

EC Number Synonyms Comment Organism
3.4.11.1 More the enzyme belongs to the peptidase family M17 Plasmodium falciparum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.1 37
-
 assay at Plasmodium falciparum