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Literature summary extracted from

  • Ago, H.; Oda, M.; Takahashi, M.; Tsuge, H.; Ochi, S.; Katunuma, N.; Miyano, M.; Sakurai, J.
    Structural basis of the sphingomyelin phosphodiesterase activity in neutral sphingomyelinase from bacillus cereus (2006), J. Biol. Chem., 281, 16157-16167.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.4.12 hanging drop vapor diffusion method, crystal structures of Bc-SMase complexed to the functional metal ions Mg2+, Co2+, or Ca2+ are determined at 1.8 A, 1.8 A, and 1.4 A resolution, respectively Bacillus cereus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.12 F285A catalytic activity of the mutant enzyme is below the detection limit, affinity of mutant enzyme to sphingomyelin liposomes are much weaker than the wild-type enzyme Bacillus cereus
3.1.4.12 W284A catalytic activity of the mutant enzyme is below the detection limit, affinity of mutant enzyme to sphingomyelin liposomes are much weaker than the wild-type enzyme Bacillus cereus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.12 Ca2+ Km: 0.0191 mM, activation of sphingomyelinase activity in the order of decreasing efficiency Co2+, Mn2+, Mg2+, Ca2+, Sr2+ Bacillus cereus
3.1.4.12 Co2+ Km: 0.142 mM, activation of sphingomyelinase activity in the order of decreasing efficiency Co2+, Mn2+, Mg2+, Ca2+, Sr2+. There are three distinct metal ion-binding sites in a long horizontal cleft across the bc-SMase molecule Bacillus cereus
3.1.4.12 Mg2+ Km: 0.0161 mM, activation of sphingomyelinase activity in the order of decreasing efficiency Co2+, Mn2+, Mg2+, Ca2+, Sr2+ Bacillus cereus
3.1.4.12 Mn2+ Km: 0.0149 mM, activation of sphingomyelinase activity in the order of decreasing efficiency Co2+, Mn2+, Mg2+, Ca2+, Sr2+ Bacillus cereus
3.1.4.12 Sr2+ Km: 0.018 mM, activation of sphingomyelinase activity in the order of decreasing efficiency Co2+, Mn2+, Mg2+, Ca2+, Sr2+ Bacillus cereus

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.12 Bacillus cereus P11889
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.12 sphingomyelin + H2O
-
 Bacillus cereus N-acylsphingosine + choline phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.12 Bc-SMase
-
 Bacillus cereus
3.1.4.12 neutral sphingomyelinase
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 Bacillus cereus