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Literature summary extracted from

  • Honda, Y.; Kitaoka, M.
    The first glycosynthase derived from an inverting glycoside hydrolase (2006), J. Biol. Chem., 281, 1426-1431.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.156 D263A conversion of glycosynthase from converting enzyme, synthesis of some xylotriose from xylobiose + HF + xylose Halalkalibacterium halodurans
3.2.1.156 D263C conversion of glycosynthase from converting enzyme, synthesis of xylotriose from xylobiose + HF + xylose, highest glycosynthase activity of mutants tested Halalkalibacterium halodurans
3.2.1.156 D263G conversion of glycosynthase from converting enzyme, synthesis of some xylotriose from xylobiose + HF + xylose Halalkalibacterium halodurans
3.2.1.156 D263L conversion of glycosynthase from converting enzyme, synthesis of some xylotriose from xylobiose + HF + xylose Halalkalibacterium halodurans
3.2.1.156 D263N conversion of glycosynthase from converting enzyme, synthesis of xylotriose from xylobiose + HF + xylose, second highest glycosynthase activity of mutants tested Halalkalibacterium halodurans
3.2.1.156 D263P very little glycosynthase or converting activity Halalkalibacterium halodurans
3.2.1.156 D263S very little glycosynthase or converting activity Halalkalibacterium halodurans
3.2.1.156 D263T conversion of glycosynthase from converting enzyme, synthesis of some xylotriose from xylobiose + HF + xylose Halalkalibacterium halodurans
3.2.1.156 D263V conversion of glycosynthase from converting enzyme, synthesis of some xylotriose from xylobiose + HF + xylose Halalkalibacterium halodurans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.156 Halalkalibacterium halodurans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.156 alpha-xylobiosylfluoride + H2O + xylose
-
 Halalkalibacterium halodurans alpha-xylobiose + HF + xylose presence of xylose is required, some mutants in D263 also produce xylotriose ?