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Literature summary extracted from

  • Athenstaedt, K.; Daum, G.
    Tgl4p and Tgl5p, two triacylglycerol lipases of the yeast Saccharomyces cerevisiae are localized to lipid particles (2005), J. Biol. Chem., 280, 37301-37309.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 genes tgl4 and tgl5, DNA and amino acid sequence determination and analysis, expression of His-tagged isozymes in Pichia pastoris, subcellular localization analysis Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.3 lipid droplet colocalization of isozymes Tgl4p and Tgl5p Saccharomyces cerevisiae 5811
-
3.1.1.3 additional information subcellular localization analysis Saccharomyces cerevisiae
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 Mg2+
-
 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.3 additional information Saccharomyces cerevisiae the enzyme is required for mobilization of triacylglycerides stored in lipid particles, overview ?
-
 ?
3.1.1.3 additional information Saccharomyces cerevisiae BY4741 the enzyme is required for mobilization of triacylglycerides stored in lipid particles, overview ?
-
 ?
3.1.1.3 trimyristin + H2O Saccharomyces cerevisiae
-
 dimyristin + myristate
-
 ?
3.1.1.3 trimyristin + H2O Saccharomyces cerevisiae BY4741
-
 dimyristin + myristate
-
 ?
3.1.1.3 tripalmitin + H2O Saccharomyces cerevisiae
-
 dipalmitin + palmitate
-
 ?
3.1.1.3 tripalmitin + H2O Saccharomyces cerevisiae BY4741
-
 dipalmitin + palmitate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Saccharomyces cerevisiae
-
 isozymes Tgl4p and Tgl5p
-
3.1.1.3 Saccharomyces cerevisiae BY4741
-
 isozymes Tgl4p and Tgl5p
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 recombinant HIs-tagged isozymes Tgl4p and Tgl5p from Pichia pastoris Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 1,2,3-trihexaicosanoylglycerol + H2O
-
 Saccharomyces cerevisiae 1,2-dihexaicosanoylglycerol + hexaicosanoate
-
 ?
3.1.1.3 additional information substrate specificity of isozymes, overview Saccharomyces cerevisiae ?
-
 ?
3.1.1.3 additional information the enzyme is required for mobilization of triacylglycerides stored in lipid particles, overview Saccharomyces cerevisiae ?
-
 ?
3.1.1.3 additional information substrate specificity of isozymes, overview Saccharomyces cerevisiae BY4741 ?
-
 ?
3.1.1.3 additional information the enzyme is required for mobilization of triacylglycerides stored in lipid particles, overview Saccharomyces cerevisiae BY4741 ?
-
 ?
3.1.1.3 trilinolein + H2O
-
 Saccharomyces cerevisiae dilinolein + linoleate
-
 ?
3.1.1.3 trimyristin + H2O
-
 Saccharomyces cerevisiae dimyristin + myristate
-
 ?
3.1.1.3 trimyristin + H2O
-
 Saccharomyces cerevisiae BY4741 dimyristin + myristate
-
 ?
3.1.1.3 triolein + H2O
-
 Saccharomyces cerevisiae diolein + oleate
-
 ?
3.1.1.3 triolein + H2O
-
 Saccharomyces cerevisiae BY4741 diolein + oleate
-
 ?
3.1.1.3 tripalmitin + H2O
-
 Saccharomyces cerevisiae dipalmitin + palmitate
-
 ?
3.1.1.3 tripalmitin + H2O
-
 Saccharomyces cerevisiae BY4741 dipalmitin + palmitate
-
 ?
3.1.1.3 tristearin + H2O
-
 Saccharomyces cerevisiae distearin + stearate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 TAG lipase
-
 Saccharomyces cerevisiae
3.1.1.3 Tgl4p
-
 Saccharomyces cerevisiae
3.1.1.3 Tgl5p
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 30
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8
-
 assay at Saccharomyces cerevisiae