Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fujiwara, K.; Toma, S.; Okamura-Ikeda, K.; Motokawa, Y.; Nakagawa, A.; Taniguchi, H.
    Crystal structure of lipoate-protein ligase A from Escherichia coli. Determination of the lipoic acid-binding site (2005), J. Biol. Chem., 280, 33645-33651.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.1.20 alone and in complex with lipoic acid Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.3.1.20 R140A 12fold increase in Km-value for apoH-protein Escherichia coli
6.3.1.20 S72A great increase in Km-value for ATP Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.20 0.00055
-
 R-lipoic acid mutant R140A, pH 7.0, 37°C Escherichia coli
6.3.1.20 0.0011
-
 apoH-protein mutant S72A, pH 7.0, 37°C Escherichia coli
6.3.1.20 0.0012
-
 apoH-protein wild-type, pH 7.0, 37°C Escherichia coli
6.3.1.20 0.0045
-
 R-lipoic acid wild-type, pH 7.0, 37°C Escherichia coli
6.3.1.20 0.0089
-
 R-lipoic acid mutant S72A, pH 7.0, 37°C Escherichia coli
6.3.1.20 0.0137
-
 ATP mutant R140A, pH 7.0, 37°C Escherichia coli
6.3.1.20 0.0155
-
 apoH-protein mutant R140A, pH 7.0, 37°C Escherichia coli
6.3.1.20 0.0158
-
 ATP wild-type, pH 7.0, 37°C Escherichia coli
6.3.1.20 0.295
-
 ATP mutant S72A, pH 7.0, 37°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.20 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.20 ATP + (R)-lipoic acid + Escherichia coli apoH-protein
-
 Escherichia coli diphosphate + AMP + Escherichia coli (R)-lipoyl-apoH-protein
-
 ?
6.3.1.20 ATP + lipoate + apoH-protein
-
 Escherichia coli ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.1.20 0.006
-
 R-lipoic acid mutant R140A, pH 7.0, 37°C Escherichia coli
6.3.1.20 0.0084
-
 ATP mutant R140A, pH 7.0, 37°C Escherichia coli
6.3.1.20 0.028
-
 apoH-protein mutant R140A, pH 7.0, 37°C Escherichia coli
6.3.1.20 0.133
-
 ATP wild-type, pH 7.0, 37°C Escherichia coli
6.3.1.20 0.16
-
 ATP mutant S72A, pH 7.0, 37°C Escherichia coli
6.3.1.20 0.178
-
 R-lipoic acid mutant S72A, pH 7.0, 37°C Escherichia coli
6.3.1.20 0.212
-
 apoH-protein mutant S72A, pH 7.0, 37°C Escherichia coli
6.3.1.20 0.219
-
 R-lipoic acid wild-type, pH 7.0, 37°C Escherichia coli
6.3.1.20 0.258
-
 apoH-protein wild-type, pH 7.0, 37°C Escherichia coli