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Literature summary extracted from

  • Savolainen, K.; Bhaumik, P.; Schmitz, W.; Kotti, T.J.; Conzelmann, E.; Wierenga, R.K.; Hiltunen, J.K.
    alpha-Methylacyl-CoA racemase from Mycobacterium tuberculosis. Mutational and structural characterization of the active site and the fold (2005), J. Biol. Chem., 280, 12611-12620.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.99.4 expression in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.1.99.4 crystal structure at 1.8 A resolution Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
5.1.99.4 C297A mutant with 6.2% relative enzyme activity Mycobacterium tuberculosis
5.1.99.4 D127A mutant with 21.6% relative enzyme activity Mycobacterium tuberculosis
5.1.99.4 D156A mutant with 17.6% relative enzyme activity Mycobacterium tuberculosis
5.1.99.4 D185A mutant with 68.5% relative enzyme activity Mycobacterium tuberculosis
5.1.99.4 D190A mutant with 3.3% relative enzyme activity Mycobacterium tuberculosis
5.1.99.4 D231A mutant with 25.0% relative enzyme activity Mycobacterium tuberculosis
5.1.99.4 D28A mutant with 24.2% relative enzyme activity Mycobacterium tuberculosis
5.1.99.4 D60A mutant with 22.7% relative enzyme activity Mycobacterium tuberculosis
5.1.99.4 D78A mutant with 44.0% relative enzyme activity Mycobacterium tuberculosis
5.1.99.4 E241A mutant with 2.1% relative enzyme activity Mycobacterium tuberculosis
5.1.99.4 E306A mutant with 23.1% relative enzyme activity Mycobacterium tuberculosis
5.1.99.4 E82A mutant with 12.5% relative enzyme activity Mycobacterium tuberculosis
5.1.99.4 E90A mutant with 67.0% relative enzyme activity Mycobacterium tuberculosis
5.1.99.4 E97A mutant with 95.4% relative enzyme activity Mycobacterium tuberculosis
5.1.99.4 H126A mutant with 4.5% relative enzyme activity Mycobacterium tuberculosis
5.1.99.4 H312A mutant with 10.1% relative enzyme activity Mycobacterium tuberculosis
5.1.99.4 I56P mutant with 28.8% relative enzyme activity Mycobacterium tuberculosis
5.1.99.4 K62A mutant with 117.6% relative enzyme activity Mycobacterium tuberculosis
5.1.99.4 M111P mutant with 5.2% relative enzyme activity Mycobacterium tuberculosis
5.1.99.4 R175A mutant with 63.6% relative enzyme activity Mycobacterium tuberculosis
5.1.99.4 R38A mutant with 20.9% relative enzyme activity Mycobacterium tuberculosis
5.1.99.4 R52A mutant with 15.7% relative enzyme activity Mycobacterium tuberculosis
5.1.99.4 R54A mutant with 37.4% relative enzyme activity Mycobacterium tuberculosis
5.1.99.4 R85A mutant with 20.3% relative enzyme activity Mycobacterium tuberculosis
5.1.99.4 R91A mutant with 19.9% relative enzyme activity, 9fold increase of Km compared with the wild type Mycobacterium tuberculosis
5.1.99.4 Y130A mutant with 24.7% relative enzyme activity Mycobacterium tuberculosis
5.1.99.4 Y224A mutant with 22.6% relative enzyme activity Mycobacterium tuberculosis
5.1.99.4 Y227A mutant with 20.1% relative enzyme activity Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.99.4 additional information
-
 additional information similar Km values for mutants H126A, D156A and E241A as for the wild-type Mycobacterium tuberculosis
5.1.99.4 0.041
-
 (R)-pristanoyl-CoA wild type Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.99.4 39000
-
 2 * 39000, SDS-PAGE Mycobacterium tuberculosis
5.1.99.4 89000
-
 gel filtration Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
5.1.99.4 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.99.4
-
 Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.99.4 additional information
-
 Vmax 214 units/mg wild-type, less than 0.1 unit/mg mutants H126A, D156A and E241A Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.99.4 (R)-pristanoyl-CoA
-
 Mycobacterium tuberculosis (S)-pristanoyl-CoA
-
 ?
5.1.99.4 (S)-2-Methylmyristoyl-CoA
-
 Mycobacterium tuberculosis (R)-2-Methylmyristoyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.99.4 dimer 2 * 39000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
5.1.99.4 AMACR
-
 Mycobacterium tuberculosis