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Literature summary extracted from

  • Sivendran, S.; Patterson, D.; Spiegel, E.; McGown, I.; Cowley, D.; Colman, R.F.
    Two novel mutant human adenylosuccinate lyases (ASLs) associated with autism and characterization of the equivalent mutant Bacillus subtilis ASL (2004), J. Biol. Chem., 279, 53789-53797.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.2.2
-
 Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
4.3.2.2 D65R mutant with differences in thermal stability, catalytic activity and substrate binding Bacillus subtilis
4.3.2.2 D65R/D69E mutant with differences in thermal stability, catalytic activity and substrate binding Bacillus subtilis
4.3.2.2 D69E mutant with differences in thermal stability, catalytic activity and substrate binding Bacillus subtilis
4.3.2.2 E80D/D87E australian patient with autism Homo sapiens
4.3.2.2 I62D mutant with differences in thermal stability, catalytic activity and substrate binding Bacillus subtilis
4.3.2.2 I62D/D65R mutant with differences in thermal stability, catalytic activity and substrate binding Bacillus subtilis
4.3.2.2 I62D/D65R/D69E mutant with differences in thermal stability, catalytic activity and substrate binding Bacillus subtilis
4.3.2.2 I62D/D69E mutant with differences in thermal stability, catalytic activity and substrate binding Bacillus subtilis
4.3.2.2 I62E mutant with differences in thermal stability, catalytic activity and substrate binding Bacillus subtilis
4.3.2.2 I62E/D65R "humanized" normal enzyme Bacillus subtilis
4.3.2.2 I62E/D65R/D69E mutant with differences in thermal stability, catalytic activity and substrate binding Bacillus subtilis
4.3.2.2 I62E/D69E mutant with differences in thermal stability, catalytic activity and substrate binding Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.2.2 0.0013
-
 adenylsuccinate mutant D69E, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
4.3.2.2 0.002
-
 adenylsuccinate mutant D65R/D69E, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
4.3.2.2 0.004
-
 adenylsuccinate 50 mM HEPES, pH 7.0, 25°C Homo sapiens
4.3.2.2 0.0043
-
 adenylsuccinate mutant I62E/D69E, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
4.3.2.2 0.0046
-
 adenylsuccinate mutant D65R, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
4.3.2.2 0.0061
-
 adenylsuccinate mutant I62E/D65R/D69E, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
4.3.2.2 0.0107
-
 adenylsuccinate mutant I62D/D65R/D69E, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
4.3.2.2 0.0175
-
 adenylsuccinate mutant I62E, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
4.3.2.2 0.0177
-
 adenylsuccinate mutant I62D/D69E, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
4.3.2.2 0.0181
-
 adenylsuccinate mutant I62E/D65R, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
4.3.2.2 0.0488
-
 adenylsuccinate mutant I62D/D65R, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
4.3.2.2 0.0551
-
 adenylsuccinate mutant I62D, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.2.2 155000
-
 mutant D69E, light scattering method, 0.25 mg/ml Bacillus subtilis
4.3.2.2 159000
-
 mutant I62E/D65R/D69E, light scattering method, 0.25 mg/ml Bacillus subtilis
4.3.2.2 173000
-
 mutant I62E, light scattering method, 0.25 mg/ml Bacillus subtilis
4.3.2.2 176000
-
 mutant I62D, light scattering method, 0.25 mg/ml Bacillus subtilis
4.3.2.2 181000
-
 light scattering method, 0.25 mg/ml Homo sapiens
4.3.2.2 209000
-
 mutant I62E/D65R, native PAGE Bacillus subtilis
4.3.2.2 210000
-
 native PAGE Homo sapiens
4.3.2.2 217000
-
 mutant I62D/D65R, native PAGE Bacillus subtilis
4.3.2.2 234000
-
 mutant I62D/D65R/D69E, light scattering method, 0.25 mg/ml Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.2.2 5-aminoimidazole-4-(N-succinylcarboxamide) ribonucleotide Bacillus subtilis
-
 5-aminoimidazole-4-carboxamide ribonucleotide + fumarate
-
 ?
4.3.2.2 5-aminoimidazole-4-(N-succinylcarboxamide) ribonucleotide Homo sapiens
-
 5-aminoimidazole-4-carboxamide ribonucleotide + fumarate
-
 ?
4.3.2.2 adenylosuccinate Bacillus subtilis
-
 AMP + fumarate
-
 ?
4.3.2.2 adenylosuccinate Homo sapiens
-
 AMP + fumarate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.2.2 Bacillus subtilis
-
-
-
4.3.2.2 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.2.2
-
 Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.2.2 0.063
-
 mutant I62D/D65R, adenylsuccinate, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
4.3.2.2 0.069
-
 mutant I62D/D65R, succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
4.3.2.2 0.072
-
 mutant I62D/D65R/D69E, adenylsuccinate, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
4.3.2.2 0.094
-
 mutant I62D/D65R/D69E, succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
4.3.2.2 0.18
-
 mutant I62D/D65R + I62E/D65R/D69E, adenylsuccinate, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
4.3.2.2 0.24
-
 mutant I62D/D65R + I62E/D65R/D69E, succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
4.3.2.2 0.33
-
 mutant I62E/D65R/D69E, adenylsuccinate, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
4.3.2.2 0.46
-
 mutant I62E/D65R/D69E, succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
4.3.2.2 0.53
-
 mutant I62E/D65R/D69E, succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
4.3.2.2 0.54
-
 mutant I62E/D65R, adenylsuccinate, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
4.3.2.2 0.97
-
 mutant I62E/D65R/D69E, adenylsuccinate + succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
4.3.2.2 1.07
-
 adenylsuccinate and succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C Homo sapiens
4.3.2.2 1.09
-
 mutant I62D/D65R, adenylsuccinate + succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
4.3.2.2 1.29
-
 mutant I62D/D65R + I62E/D65R/D69E, adenylsuccinate + succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
4.3.2.2 1.3
-
 mutant I62D/D65R/D69E, adenylsuccinate + succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
4.3.2.2 1.4
-
 mutant I62E/D65R/D69E, adenylsuccinate + succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
4.3.2.2 1.58
-
 adenylsuccinate, 50 mM HEPES, pH 7.0, 25°C Homo sapiens
4.3.2.2 1.7
-
 succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.2.2 5-aminoimidazole-4-(N-succinylcarboxamide) ribonucleotide
-
 Bacillus subtilis 5-aminoimidazole-4-carboxamide ribonucleotide + fumarate
-
 ?
4.3.2.2 5-aminoimidazole-4-(N-succinylcarboxamide) ribonucleotide
-
 Homo sapiens 5-aminoimidazole-4-carboxamide ribonucleotide + fumarate
-
 ?
4.3.2.2 adenylosuccinate
-
 Bacillus subtilis AMP + fumarate
-
 ?
4.3.2.2 adenylosuccinate
-
 Homo sapiens AMP + fumarate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.2.2 homotetramer
-
 Bacillus subtilis
4.3.2.2 homotetramer
-
 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
4.3.2.2 ASL
-
 Bacillus subtilis
4.3.2.2 ASL
-
 Homo sapiens