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Literature summary extracted from

  • Eastmond, P.J.
    Cloning and characterization of the acid lipase from castor beans (2004), J. Biol. Chem., 279, 45540-45545.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.3 additional information no activation by NaCl Ricinus communis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 DNA and amino acid sequence determination and analysis, expression in Escherichia coli Ricinus communis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 4-chloromercuribenzoate
-
 Ricinus communis
3.1.1.3 diethyl 4-nitrophenyl phosphate
-
 Ricinus communis
3.1.1.3 EDTA slight inhibition Ricinus communis
3.1.1.3 HgCl2
-
 Ricinus communis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.3 oil body associated to the surface of the oil body membrane in endosperm Ricinus communis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 Ca2+ slight activation Ricinus communis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 60000
-
 x * 60000, about, SDS-PAGE Ricinus communis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.3 additional information Ricinus communis the enzyme is involved in regulation of lipolysis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Ricinus communis Q5VKJ7
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.3 triacylglycerol + H2O = diacylglycerol + a carboxylate the enzyme contains an active site serine residue Ricinus communis
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.3 endosperm restricted to Ricinus communis
-
3.1.1.3 additional information no expression in mature leaf, stem, and root, the enzyme expression decreases during germination, developmental and tissue-specific distribution Ricinus communis
-
3.1.1.3 seed
-
 Ricinus communis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 0.0034
-
 purified recombinant enzyme, substrate triplamitin Ricinus communis
3.1.1.3 0.0054
-
 purified recombinant enzyme, substrate triolein Ricinus communis
3.1.1.3 0.0058
-
 purified recombinant enzyme, substrate trilinolein Ricinus communis
3.1.1.3 0.0073
-
 purified recombinant enzyme, substrate triricinolein Ricinus communis
3.1.1.3 0.0089
-
 purified recombinant enzyme, substrate trilaurin Ricinus communis
3.1.1.3 0.0214
-
 purified recombinant enzyme, substrate tricaprin Ricinus communis
3.1.1.3 0.0242
-
 purified recombinant enzyme, substrate 4-nitrophenyl butyrate Ricinus communis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl butyrate + H2O high activity Ricinus communis 4-nitrophenol + butyrate
-
 ?
3.1.1.3 4-nitrophenyl laurate + H2O
-
 Ricinus communis 4-nitrophenol + laurate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O low activity Ricinus communis 4-nitrophenol + palmitate
-
 ?
3.1.1.3 additional information the enzyme is involved in regulation of lipolysis Ricinus communis ?
-
 ?
3.1.1.3 additional information substrate specificity, no activity with oleoyl-CoA and 1,2-dioleoyl-3-phosphatidylcholine Ricinus communis ?
-
 ?
3.1.1.3 tricaprin + H2O
-
 Ricinus communis dicaprin + caprate
-
 ?
3.1.1.3 trilaurin + H2O
-
 Ricinus communis dilaurin + laurate
-
 ?
3.1.1.3 trilinolein + H2O
-
 Ricinus communis dilinolein + linoleate
-
 ?
3.1.1.3 triolein + H2O
-
 Ricinus communis diolein + oleate
-
 ?
3.1.1.3 tripalmitin + H2O
-
 Ricinus communis dipalmitin + palmitate
-
 ?
3.1.1.3 triricinolein + H2O
-
 Ricinus communis diricinolein + ricinolate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 60000, about, SDS-PAGE Ricinus communis

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 acid lipase
-
 Ricinus communis
3.1.1.3 OBL1
-
 Ricinus communis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 30
-
 assay at Ricinus communis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 4.5
-
 recombinant enzyme Ricinus communis