Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chi, Y.; Zhou, B.; Wang, W.Q.; Chung, S.K.; Kwon, Y.U.; Ahn, Y.H.; Chang, Y.T.; Tsujishita, Y.; Hurley, J.H.; Zhang, Z.Y.
    Comparative mechanistic and substrate specificity study of inositol polyphosphate 5-phosphatase Schizosaccharomyces pombe Synaptojanin and SHIP2 (2004), J. Biol. Chem., 279, 44987-44995.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.36 expression of His-tagged full length enzyme in Escherichia coli strain BL21(DE3) Schizosaccharomyces pombe
3.1.3.56 expression of His-tagged full length enzyme in Escherichia coli strain BL21(DE3) Schizosaccharomyces pombe
3.1.3.56 expression of His-tagged isolated catalytic domain in Escherichia coli strain BL21(DE3) Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.36 E597Q site-directed mutagenesis, the mutant enzyme shows an over 140fold increased catalytic efficiency and a 2.4fold reduced affinity for Mg2+ compared to the wild-type enzyme Schizosaccharomyces pombe
3.1.3.56 E597Q site-directed mutagenesis, the mutant enzyme shows an over 140fold increased catalytic efficiency and a 2.4fold reduced affinity for Mg2+ compared to the wild-type enzyme Schizosaccharomyces pombe

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.36 Ca2+ competitive to Mg2+ Schizosaccharomyces pombe
3.1.3.36 Cu2+
-
 Schizosaccharomyces pombe
3.1.3.56 Ca2+ competitive to Mg2+ Schizosaccharomyces pombe
3.1.3.56 Cu2+
-
 Schizosaccharomyces pombe

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.36 additional information
-
 additional information kinetics, substrate specificity, overview Schizosaccharomyces pombe
3.1.3.36 0.056
-
 7-methyl-6-thioguanosine pH 7.0, 30°C, recombinant enzyme Schizosaccharomyces pombe
3.1.3.56 additional information
-
 additional information kinetics, substrate specificity, overview Homo sapiens
3.1.3.56 additional information
-
 additional information kinetics, substrate specificity, overview Schizosaccharomyces pombe
3.1.3.56 0.056
-
 7-methyl-6-thioguanosine pH 7.0, 30°C, recombinant enzyme Schizosaccharomyces pombe

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.36 Co2+ activates, competitive binding to other metal ions, KM 0.0145 mM and turnover number 7.28 s-1 at pH 7.0, 30°C, recombinant enzyme Schizosaccharomyces pombe
3.1.3.36 Mg2+ required, optimal at 2 mM, existence of a second inhibitory metal binding site, competitive binding to other metal ions, KM 0.53 mM and turnover number 9.64 s-1 at pH 7.0, 30°C, recombinant enzyme Schizosaccharomyces pombe
3.1.3.36 Mn2+ activates, competitive binding to other metal ions, KM 0.012 mM and turnover number 7.48 s-1 at pH 7.0, 30°C, recombinant enzyme Schizosaccharomyces pombe
3.1.3.36 Ni2+ activates, competitive binding to other metal ions, KM 0.0064 mM and turnover number 5.48 s-1 at pH 7.0, 30°C, recombinant enzyme Schizosaccharomyces pombe
3.1.3.56 Co2+ activates, competitive binding to other metal ions, KM 0.0145 mM and turnover number 7.28 s-1 at pH 7.0, 30°C, recombinant enzyme Schizosaccharomyces pombe
3.1.3.56 Mg2+
-
 Homo sapiens
3.1.3.56 Mg2+ required, optimal at 2 mM, existence of a second inhibitory metal binding site, competitive binding to other metal ions, KM 0.53 mM and turnover number 9.64 s-1 at pH 7.0, 30°C, recombinant enzyme Schizosaccharomyces pombe
3.1.3.56 Mn2+ activates, competitive binding to other metal ions, KM 0.012 mM and turnover number 7.48 s-1 at pH 7.0, 30°C, recombinant enzyme Schizosaccharomyces pombe
3.1.3.56 Ni2+ activates, competitive binding to other metal ions, KM 0.0064 mM and turnover number 5.48 s-1 at pH 7.0, 30°C, recombinant enzyme Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.36 additional information Schizosaccharomyces pombe the enzyme has multiple roles in cellular signalling and may regulate distinct pathways ?
-
 ?
3.1.3.56 additional information Homo sapiens the enzyme has multiple roles in cellular signalling and may regulate distinct pathways ?
-
 ?
3.1.3.56 additional information Schizosaccharomyces pombe the enzyme has multiple roles in cellular signalling and may regulate distinct pathways ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.36 Schizosaccharomyces pombe
-
-
-
3.1.3.56 Homo sapiens
-
-
-
3.1.3.56 Schizosaccharomyces pombe
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.36 recombinant His-tagged full length enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Schizosaccharomyces pombe
3.1.3.56 recombinant His-tagged catalytic domain from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, the tag is cleaved off Homo sapiens
3.1.3.56 recombinant His-tagged full length enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Schizosaccharomyces pombe

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.36 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate catalytic and kinetic reaction mechanism, substrate binding, overview Schizosaccharomyces pombe
a
3.1.3.56 1D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O = 1D-myo-inositol 1,3,4-trisphosphate + phosphate catalytic and kinetic reaction mechanism, substrate binding, overview Homo sapiens
a
3.1.3.56 1D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O = 1D-myo-inositol 1,3,4-trisphosphate + phosphate catalytic and kinetic reaction mechanism, substrate binding, overview Schizosaccharomyces pombe
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.36 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + H2O
-
 Schizosaccharomyces pombe 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate
-
 ?
3.1.3.36 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate + H2O
-
 Schizosaccharomyces pombe 1-phosphatidyl-1D-myo-inositol 3-phosphate + phosphate
-
 ?
3.1.3.36 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
-
 Schizosaccharomyces pombe 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate
-
 ?
3.1.3.36 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + H2O
-
 Schizosaccharomyces pombe 1D-myo-inositol 1,2,4,6-tetrakisphosphate + phosphate
-
 ?
3.1.3.36 1D-myo-inositol 1,2,4,5-tetrakisphosphate + H2O
-
 Schizosaccharomyces pombe 1D-myo-inositol 1,2,4-trisphosphate + phosphate
-
 ?
3.1.3.36 1D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O
-
 Schizosaccharomyces pombe 1D-myo-inositol 1,3,4-trisphosphate + phosphate
-
 ?
3.1.3.36 1D-myo-inositol 1,4,5-trisphosphate + H2O
-
 Schizosaccharomyces pombe 1D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?
3.1.3.36 1D-myo-inositol 2,4,5,6-tetrakisphosphate + H2O
-
 Schizosaccharomyces pombe 1D-myo-inositol 2,4,6-trisphosphate + phosphate
-
 ?
3.1.3.36 1D-myo-inositol 2,4,5-trisphosphate + H2O preferred substrate Schizosaccharomyces pombe 1D-myo-inositol 2,4-bisphosphate + phosphate
-
 ?
3.1.3.36 1D-myo-inositol 4,5,6-trisphosphate + H2O
-
 Schizosaccharomyces pombe 1D-myo-inositol 4,6-bisphosphate + phosphate
-
 ?
3.1.3.36 3-O-methylfluorescein phosphate + H2O
-
 Schizosaccharomyces pombe 3-O-methylfluorescein + phosphate
-
 ?
3.1.3.36 4-nitrophenyl phosphate + H2O
-
 Schizosaccharomyces pombe 4-nitrophenol + phosphate
-
 ?
3.1.3.36 7-methyl-6-thioguanosine + H2O spectrophotometric continuous coupled enzyme assay substrate Schizosaccharomyces pombe 7-methyl-6-thioguanine + ribose 1-phosphate
-
 ?
3.1.3.36 additional information the enzyme has multiple roles in cellular signalling and may regulate distinct pathways Schizosaccharomyces pombe ?
-
 ?
3.1.3.36 additional information substrate specificity, no activity with 1-phosphatidyl-1D-myo-inositol 5-phosphate, 1D-myo-inositol 1,5-bisphosphate, 1D-myo-inositol 1,3,5-trisphosphate, and with alpha-D-glucose 1-phosphate, D-glucose 6-phosphate, and alpha-D,L-glycerophosphate, overview Schizosaccharomyces pombe ?
-
 ?
3.1.3.56 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + H2O
-
 Homo sapiens 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate
-
 ?
3.1.3.56 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + H2O
-
 Schizosaccharomyces pombe 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate
-
 ?
3.1.3.56 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate + H2O
-
 Homo sapiens 1-phosphatidyl-1D-myo-inositol 3-phosphate + phosphate
-
 ?
3.1.3.56 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate + H2O
-
 Schizosaccharomyces pombe 1-phosphatidyl-1D-myo-inositol 3-phosphate + phosphate
-
 ?
3.1.3.56 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
-
 Schizosaccharomyces pombe 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate
-
 ?
3.1.3.56 1D-myo-inositol 1,2,3,4,5-pentakisphosphate + H2O preferred substrate Homo sapiens 1D-myo-inositol 1,2,3,4-tetrakisphosphate + phosphate
-
 ?
3.1.3.56 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + H2O
-
 Schizosaccharomyces pombe 1D-myo-inositol 1,2,4,6-tetrakisphosphate + phosphate
-
 ?
3.1.3.56 1D-myo-inositol 1,2,4,5-tetrakisphosphate + H2O
-
 Schizosaccharomyces pombe 1D-myo-inositol 1,2,4-trisphosphate + phosphate
-
 ?
3.1.3.56 1D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O
-
 Homo sapiens 1D-myo-inositol 1,3,4-trisphosphate + phosphate
-
 ?
3.1.3.56 1D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O
-
 Schizosaccharomyces pombe 1D-myo-inositol 1,3,4-trisphosphate + phosphate
-
 ?
3.1.3.56 1D-myo-inositol 1,4,5,6-tetrakisphosphate + H2O
-
 Homo sapiens 1D-myo-inositol 1,4,6-trisphosphate + phosphate
-
 ?
3.1.3.56 1D-myo-inositol 1,4,5-trisphosphate + H2O
-
 Schizosaccharomyces pombe 1D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?
3.1.3.56 1D-myo-inositol 2,4,5,6-tetrakisphosphate + H2O
-
 Schizosaccharomyces pombe 1D-myo-inositol 2,4,6-trisphosphate + phosphate
-
 ?
3.1.3.56 1D-myo-inositol 2,4,5,6-tetrakisphosphate + H2O low activity Homo sapiens 1D-myo-inositol 2,4,6-trisphosphate + phosphate
-
 ?
3.1.3.56 1D-myo-inositol 2,4,5-trisphosphate + H2O preferred substrate Schizosaccharomyces pombe 1D-myo-inositol 2,4-bisphosphate + phosphate
-
 ?
3.1.3.56 1D-myo-inositol 4,5,6-trisphosphate + H2O
-
 Schizosaccharomyces pombe 1D-myo-inositol 4,6-bisphosphate + phosphate
-
 ?
3.1.3.56 3-O-methylfluorescein phosphate + H2O
-
 Schizosaccharomyces pombe 3-O-methylfluorescein + phosphate
-
 ?
3.1.3.56 4-nitrophenyl phosphate + H2O
-
 Schizosaccharomyces pombe 4-nitrophenol + phosphate
-
 ?
3.1.3.56 7-methyl-6-thioguanosine + H2O spectrophotometric continous coupled enzyme assay substrate Schizosaccharomyces pombe 7-methyl-6-thioguanine + ribose 1-phosphate
-
 ?
3.1.3.56 additional information the enzyme has multiple roles in cellular signalling and may regulate distinct pathways Homo sapiens ?
-
 ?
3.1.3.56 additional information the enzyme has multiple roles in cellular signalling and may regulate distinct pathways Schizosaccharomyces pombe ?
-
 ?
3.1.3.56 additional information substrate specificity, no activity with 1-phosphatidyl-1D-myo-inositol 5-phosphate, 1D-myo-inositol 1,5-bisphosphate, 1D-myo-inositol 1,3,5-trisphosphate, and with alpha-D-glucose 1-phosphate, D-glucose 6-phosphate, and alpha-D,L-glycerophosphate, overview Schizosaccharomyces pombe ?
-
 ?
3.1.3.56 additional information substrate specificity, no activity with 1D-myo-inositol 1,4,5-trisphosphate, 1-phosphatidyl-1D-myo-inositol 5-phosphate, 1D-myo-inositol 1,5-bisphosphate, and 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate, overview Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.36 More three-dimensional structure analysis of enzyme with active site-bound substrate, analysis of crystal structure Schizosaccharomyces pombe
3.1.3.56 More three-dimensional structure analysis of enzyme with active site-bound substrate, analysis of crystal structure Schizosaccharomyces pombe

Synonyms

EC Number Synonyms Comment Organism
3.1.3.36 More cf. EC 3.1.3.56, inositol polyphosphate 5-phosphatase Schizosaccharomyces pombe
3.1.3.36 SPsynaptojanin
-
 Schizosaccharomyces pombe
3.1.3.36 Synaptojanin
-
 Schizosaccharomyces pombe
3.1.3.56 inositol polyphosphate 5-phosphatase
-
 Homo sapiens
3.1.3.56 inositol polyphosphate 5-phosphatase
-
 Schizosaccharomyces pombe
3.1.3.56 More cf. EC 3.1.3.36, phosphoinositide 5-phosphatase Schizosaccharomyces pombe
3.1.3.56 SHIP2
-
 Homo sapiens
3.1.3.56 SPsynaptojanin
-
 Schizosaccharomyces pombe
3.1.3.56 Synaptojanin
-
 Schizosaccharomyces pombe

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.36 30
-
 assay at Schizosaccharomyces pombe
3.1.3.56 30
-
 assay at Homo sapiens
3.1.3.56 30
-
 assay at Schizosaccharomyces pombe

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.36 11
-
 7-methyl-6-thioguanosine pH 7.0, 30°C, recombinant enzyme Schizosaccharomyces pombe
3.1.3.56 11
-
 7-methyl-6-thioguanosine pH 7.0, 30°C, recombinant enzyme Schizosaccharomyces pombe

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.36 7 7.4 assay at Schizosaccharomyces pombe
3.1.3.56 7 7.4 assay at Homo sapiens
3.1.3.56 7 7.4 assay at Schizosaccharomyces pombe

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.36 additional information
-
 pH profile Schizosaccharomyces pombe
3.1.3.56 additional information
-
 pH profile Schizosaccharomyces pombe

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.36 additional information
-
 additional information
-
 Schizosaccharomyces pombe
3.1.3.56 additional information
-
 additional information
-
 Schizosaccharomyces pombe