EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.2 | Tartrate | slight activation | Phaseolus vulgaris |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.2 | gene KeACP, DNA and amino acid sequence determination and analysis | Phaseolus vulgaris |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.2 | molybdate | complete inhibition at 0.1 mM | Phaseolus vulgaris | |
3.1.3.2 | vanadate | 30 min, 30°C, leads to complete loss of phosphatase activity of the enzyme at 0.1 mM and gain of chloroperoxidase activity by the enzyme, overview | Phaseolus vulgaris |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.2 | additional information | the enzyme is not affected by citrate, EDTA, fluoride, Zn2+, Fe3+, and Mn2+ | Phaseolus vulgaris |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.3.2 | 45000 | - |
1 * 45000 + 1 * 56000, SDS-PAGE | Phaseolus vulgaris |
3.1.3.2 | 56000 | - |
1 * 45000 + 1 * 56000, SDS-PAGE | Phaseolus vulgaris |
3.1.3.2 | 96000 | - |
gel filtration | Phaseolus vulgaris |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.2 | Phaseolus vulgaris | Q764C1 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.1.3.2 | glycoprotein | both subunit types are glycosylated | Phaseolus vulgaris |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.2 | 852fold to homogeneity from embryonic axes by DEAE ion exchange and concanavalin A affinity chromatography | Phaseolus vulgaris |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.3.2 | embryo | expression during elongation in germination | Phaseolus vulgaris | - |
3.1.3.2 | seed | embryonic axes, expression during elongation in germination | Phaseolus vulgaris | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.3.2 | 383.3 | - |
purified enzyme | Phaseolus vulgaris |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.2 | 2-phosphoglycerate + H2O | - |
Phaseolus vulgaris | glycerate + phosphate | - |
? | |
3.1.3.2 | 3-phosphoglycerate + H2O | - |
Phaseolus vulgaris | glycerate + phosphate | - |
? | |
3.1.3.2 | 4-nitrophenyl phosphate + H2O | - |
Phaseolus vulgaris | 4-nitrophenol + phosphate | - |
? | |
3.1.3.2 | ADP + H2O | low activity | Phaseolus vulgaris | AMP + phosphate | - |
? | |
3.1.3.2 | alpha-naphthyl phosphate + H2O | - |
Phaseolus vulgaris | alpha-naphthol + phosphate | - |
? | |
3.1.3.2 | AMP + H2O | - |
Phaseolus vulgaris | adenosine + phosphate | - |
? | |
3.1.3.2 | ATP + H2O | - |
Phaseolus vulgaris | ADP + phosphate | - |
? | |
3.1.3.2 | beta-naphthyl phosphate + H2O | - |
Phaseolus vulgaris | beta-naphthol + phosphate | - |
? | |
3.1.3.2 | D-glucose 1-phosphate + H2O | - |
Phaseolus vulgaris | D-glucose + phosphate | - |
? | |
3.1.3.2 | D-glucose 6-phosphate + H2O | - |
Phaseolus vulgaris | D-glucose + phosphate | - |
? | |
3.1.3.2 | additional information | substrate specificity, overview, vanadate leads to loss of phosphatase activity of the enzyme and gain of chloroperoxidase activity by the enzyme, overview | Phaseolus vulgaris | ? | - |
? | |
3.1.3.2 | phospho-L-serine + H2O | low activity | Phaseolus vulgaris | L-serine + phosphate | - |
? | |
3.1.3.2 | phospho-L-threonine + H2O | - |
Phaseolus vulgaris | L-threonine + phosphate | - |
? | |
3.1.3.2 | phospho-L-tyrosine + H2O | - |
Phaseolus vulgaris | L-tyrosine + phosphate | - |
? | |
3.1.3.2 | phosphoenolpyruvate + H2O | - |
Phaseolus vulgaris | pyruvate + phosphate | - |
? | |
3.1.3.2 | phytate + H2O | low activity | Phaseolus vulgaris | ? + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.3.2 | dimer | 1 * 45000 + 1 * 56000, SDS-PAGE | Phaseolus vulgaris |
3.1.3.2 | More | three combinations of the two subunit types occur with the 56 kDa subunit dominating, no intersubunit disulfide linkages | Phaseolus vulgaris |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.2 | colorless acid phosphatase | - |
Phaseolus vulgaris |
3.1.3.2 | KeACP | - |
Phaseolus vulgaris |
3.1.3.2 | More | the colorless acid phosphatase is distinct from the purple acid phosphatase | Phaseolus vulgaris |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.2 | 30 | - |
assay at | Phaseolus vulgaris |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.2 | 6 | - |
- |
Phaseolus vulgaris |