EC Number | Cloned (Comment) | Organism |
---|---|---|
4.4.1.1 | site directed mutagenesis mutants, expression in Escherichia coli | Treponema denticola |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.4.1.1 | K238A | mutant with complete loss of lyase and racemase activity | Treponema denticola |
4.4.1.1 | Y123F | mutant with decreased activity | Treponema denticola |
4.4.1.1 | Y123F/Y124F | mutant with decreased activity | Treponema denticola |
4.4.1.1 | Y124F | mutant with 50% of kcat for transamination of L- and D-alanine | Treponema denticola |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.4.1.1 | 0.1 | - |
beta-chloro-L-alanine | Y123F/Y124F mutant, 20 mM potassium phosphate buffer, pH 7.4, 25°C | Treponema denticola | |
4.4.1.1 | 0.17 | - |
beta-chloro-L-alanine | Y123F mutant, 20 mM potassium phosphate buffer, pH 7.4, 25°C | Treponema denticola | |
4.4.1.1 | 0.76 | - |
D-alanine | racemization, Y123F/Y124F mutant, 20 mM potassium phosphate buffer, pH 7.4, 25°C | Treponema denticola | |
4.4.1.1 | 1 | - |
L-alanine | racemization, Y123F/Y124F mutant, 20 mM potassium phosphate buffer, pH 7.4, 25°C | Treponema denticola | |
4.4.1.1 | 1.21 | - |
beta-chloro-L-alanine | wild type, 20 mM potassium phosphate buffer, pH 7.4, 25°C | Treponema denticola | |
4.4.1.1 | 3.2 | - |
beta-chloro-L-alanine | Y124F mutant, 20 mM potassium phosphate buffer, pH 7.4, 25°C | Treponema denticola | |
4.4.1.1 | 10 | - |
L-alanine | racemization, wild type, 20 mM potassium phosphate buffer, pH 7.4, 25°C | Treponema denticola | |
4.4.1.1 | 10 | - |
D-alanine | racemization, wild type, 20 mM potassium phosphate buffer, pH 7.4, 25°C | Treponema denticola | |
4.4.1.1 | 14.3 | - |
L-alanine | racemization, Y123F mutant, 20 mM potassium phosphate buffer, pH 7.4, 25°C | Treponema denticola | |
4.4.1.1 | 20 | - |
L-alanine | racemization, Y124F mutant, 20 mM potassium phosphate buffer, pH 7.4, 25°C | Treponema denticola | |
4.4.1.1 | 22.4 | - |
D-alanine | racemization, Y123F mutant, 20 mM potassium phosphate buffer, pH 7.4, 25°C | Treponema denticola | |
4.4.1.1 | 25.3 | - |
D-alanine | racemization, Y124F mutant, 20 mM potassium phosphate buffer, pH 7.4, 25°C | Treponema denticola |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.4.1.1 | Treponema denticola | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.4.1.1 | - |
Treponema denticola |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.4.1.1 | beta-chloro-L-alanine + H2O | - |
Treponema denticola | pyruvate + NH3 + HCl | - |
? | |
4.4.1.1 | D-alanine + H2O | - |
Treponema denticola | ? | - |
? | |
4.4.1.1 | L-alanine + H2O | - |
Treponema denticola | ? | - |
? | |
4.4.1.1 | L-cysteine + H2O | - |
Treponema denticola | sulfide + NH3 + pyruvate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.4.1.1 | homodimer | - |
Treponema denticola |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.4.1.1 | pyridoxal 5'-phosphate | enzyme binds 2 mol per dimer | Treponema denticola |