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Literature summary extracted from

  • Park, S.W.; Vepachedu, R.; Owens, R.A.; Vivanco, J.M.
    The N-glycosidase activity of the ribosome-inactivating protein ME1 targets single-stranded regions of nucleic acids independent of sequence or structural motifs (2004), J. Biol. Chem., 279, 34165-34174.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.22 Mirabilis expansa
-
 type I ribosome-inactivating protein ME1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.22 native enzyme from storage roots by immunoaffinity chromatography using a polyclonal anti-ME1 antibody resin Mirabilis expansa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.2.22 root storage root Mirabilis expansa
-
3.2.2.22 storage tissue
-
 Mirabilis expansa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.22 28S rRNA + H2O substrate is from Saccharomyces cerevisiae YPH500 ribosomes, depurination of an adenine of the 28S rRNA, substrate structure oveview Mirabilis expansa ?
-
 ?
3.2.2.22 additional information substrate specificity, no activity with double-stranded heat-denatured DNA, the N-glycosidase activity of the ribosome-inactivating protein ME1 targets single-stranded regions of nucleic acids independent of sequence or structural motifs Mirabilis expansa ?
-
 ?
3.2.2.22 non-ribosomal RNA + H2O e.g. mRNA: Phytophthora cryptotogea B26 RNA, or Arabidopsis thaliana RNA, single-stranded, identification of cleavage sites, overview Mirabilis expansa ?
-
 ?
3.2.2.22 single-stranded DNA + H2O at 90°C heat-denatured substrate Mirabilis expansa ?
-
 ?
3.2.2.22 un-capped RNA + H2O
-
 Mirabilis expansa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.22 ME1
-
 Mirabilis expansa
3.2.2.22 RNA N-glycosidase
-
 Mirabilis expansa
3.2.2.22 type I ribosome-inactivating protein
-
 Mirabilis expansa
3.2.2.22 type I RIP
-
 Mirabilis expansa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.22 30
-
 assay at Mirabilis expansa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.22 7.6
-
 assay at Mirabilis expansa