Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Anderson, K.A.; Noeldner, P.K.; Reece, K.; Wadzinski, B.E.; Means, A.R.
    Regulation and function of the calcium/calmodulin-dependent protein kinase IV/protein serine/threonine phosphatase 2A signaling complex (2004), J. Biol. Chem., 279, 31708-31716.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.17 Calmodulin dependent on, transient, tightly regulated Ca2+/calmodulin-dependent activation requiring phosphorylation at Thr200 by CaMK kinase, the Ca2+/calmodulin binding-autoinhibitory domain is required for interaction with protein phosphatase PP2A, binding of Ca2+/CaM and PP2A is exclusive, overview Mus musculus
2.7.11.17 ionomycin
-
 Mus musculus
2.7.11.17 additional information transient, tightly regulated Ca2+/calmodulin-dependent activation requiring phosphorylation at Thr200 by CaMK kinase, the Ca2+/calmodulin binding-autoinhibitory domain is required for interaction with protein phosphatase PP2A, binding of Ca2+/CaM and PP2A is exclusive, overview Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.17 expression of FLAG-tagged CaMKIV in 293A cells Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.17 T200A site-directed mutagenesis, phosphorylation site mutant of CaMKIV, no activation by phosphorylation through CaMKK, no stimulation by ionomycin in absence of Ca2+ and calmodulin Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.17 Ca2+ dependent on, transient, tightly regulated Ca2+/calmodulin-dependent activation requiring phosphorylation at Thr200 by CaMK kinase, the Ca2+/calmodulin binding-autoinhibitory domain is required for interaction with protein phosphatase PP2A, binding of Ca2+/CaM and PP2A is exclusive, overview Mus musculus
2.7.11.17 Mg2+
-
 Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.17 ATP + cAMP response element-binding protein Mus musculus CaMKIV activates CREB by phosphorylation and stimulates CREB-mediated transcription ADP + phosphorylated cAMP response element-binding protein
-
 ?
2.7.11.17 additional information Mus musculus CaMKIV regulation, overview, CaMKIV functions as a potent stimulator of Ca2+-dependent gene expression ?
-
 ?
3.1.3.16 additional information Homo sapiens regulation and function of the stably associated calcium/calmodulin-dependent protein kinase IV/protein serine/threonine phosphatase 2A signaling complex, PP2A is negatively regulating the kinase, interaction requires the Ca2+/CaM-binding autoinhibitory domain of CaMKIV ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.17 Mus musculus
-
-
-
3.1.3.16 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.17 phosphoprotein transient, tightly regulated Ca2+/calmodulin-dependent activation requiring phosphorylation at Thr200 by CaMK kinase, the Ca2+/calmodulin binding-autoinhibitory domain is required for interaction with protein phosphatase PP2A, binding of Ca2+/CaM and PP2A is exclusive, overview Mus musculus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.17 recombinant FLAG-tagged CaMKIV from 293A cells by immunoaffinity chromatography Mus musculus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.16 [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate regulation and function of the calcium/calmodulin-dependent protein kinase IV/protein serine/threonine phosphatase 2A signaling complex Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.17 brain
-
 Mus musculus
-
3.1.3.16 HEK-293A cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.17 ATP + cAMP response element-binding protein CaMKIV activates CREB by phosphorylation and stimulates CREB-mediated transcription Mus musculus ADP + phosphorylated cAMP response element-binding protein
-
 ?
2.7.11.17 ATP + cAMP response element-binding protein i.e. CREB Mus musculus ADP + phosphorylated cAMP response element-binding protein
-
 ?
2.7.11.17 ATP + PLRRTLSVAA
-
 Mus musculus ADP + ?
-
 ?
2.7.11.17 additional information CaMKIV regulation, overview, CaMKIV functions as a potent stimulator of Ca2+-dependent gene expression Mus musculus ?
-
 ?
3.1.3.16 additional information regulation and function of the stably associated calcium/calmodulin-dependent protein kinase IV/protein serine/threonine phosphatase 2A signaling complex, PP2A is negatively regulating the kinase, interaction requires the Ca2+/CaM-binding autoinhibitory domain of CaMKIV Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.17 calcium/calmodulin-dependent protein kinase IV
-
 Mus musculus
2.7.11.17 calcium/calmodulin-dependent protein kinase IV/protein serine/threonine phosphatase 2A signaling complex
-
 Mus musculus
2.7.11.17 CaMKIV
-
 Mus musculus
3.1.3.16 PP2A
-
 Homo sapiens
3.1.3.16 protein serine/threonine phosphatase 2A
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.17 30
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.17 7.4
-
 assay at Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.17 ATP
-
 Mus musculus