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Literature summary extracted from

  • Tringali, C.; Papini, N.; Fusi, P.; Croci, G.; Borsani, G.; Preti, A.; Tortora, P.; Tettamanti, G.; Venerando, B.; Monti, E.
    Properties of recombinant human cytosolic sialidase HsNEU2: The enzyme hydrolyzes monomerically dispersed GM1 ganglioside molecules (2004), J. Biol. Chem., 279, 3169-3179.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.18 octyl glucoside highest activation (1.3-2.2fold) at 0.08% w/v concentration Homo sapiens
3.2.1.18 sodium deoxycholate highest activation (1.1-1.8.5fold) at 0.03% w/v concentration Homo sapiens
3.2.1.18 Triton X-100 highest activation (1.5-2.5fold) at 0.02% w/v concentration Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.18
-
 Homo sapiens

General Stability

EC Number General Stability Organism
3.2.1.18 the enzyme is unstable upon freezing at -20°C, 50% loss of activity after one cycle of freezing-thawing Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.18 Ca2+ above 1 mM Homo sapiens
3.2.1.18 Cu2+ above 0.01 mM Homo sapiens
3.2.1.18 K+ above 1 mM Homo sapiens
3.2.1.18 Na+ above 1 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.18 0.14
-
 ganglioside GD1a 37°C, pH 5.6 or 7.0 activated by 0.02% Triton X-100 Homo sapiens
3.2.1.18 0.24
-
 ganglioside GM3 37°C, pH 5.6 or 7.0, activated by 0.02% Triton X-100 Homo sapiens
3.2.1.18 0.28
-
 alpha(2-3)sialylparagloboside 37°C, pH 5.6 or 7.0 Homo sapiens
3.2.1.18 0.31
-
 alpha(2-3)sialyllactose 37°C, pH 5.6 or 7.0 Homo sapiens
3.2.1.18 0.38
-
 ganglioside GT1b 37°C, pH 5.6 or 7.0 activated by 0.02% Triton X-100 Homo sapiens
3.2.1.18 0.4
-
 ganglioside GM3 37°C, pH 5.6 or 7.0, without Triton X-100 Homo sapiens
3.2.1.18 0.43
-
 alpha(2-3)sialylparagloboside 37°C, pH 5.6 or 7.0 Homo sapiens
3.2.1.18 0.44
-
 4-methylumbelliferyl alpha-NeuAc 37°C, pH 5.6 or 7.0 Homo sapiens
3.2.1.18 0.51
-
 ganglioside GD1b 37°C, pH 5.6 or 7.0 activated by 0.02% Triton X-100 Homo sapiens
3.2.1.18 0.59
-
 ganglioside GD1b 37°C, pH 5.6 or 7.0, without Triton X-100 Homo sapiens
3.2.1.18 0.62
-
 ganglioside GT1b 37°C, pH 5.6 or 7.0, without Triton X-100 Homo sapiens
3.2.1.18 0.91
-
 ganglioside GD1a 37°C, pH 5.6 or 7.0, without Triton X-100 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.18 cytosol
-
 Homo sapiens 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.18 K+ slight activation up to 0.01 mM Homo sapiens
3.2.1.18 Na+ slight activation up to 0.01 mM Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.18 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.18 recombinant Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.2.1.18 -80°C, stable for 1 year Homo sapiens
3.2.1.18 4°C, 50% loss of activity after 24 h Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.18 4-methylumbelliferyl alpha-NeuAc + H2O
-
 Homo sapiens 4-methylumbelliferone + alpha-neuAc
-
 ?
3.2.1.18 alpha(2-3)sialyllactose + H2O
-
 Homo sapiens sialic acid + lactose
-
 ?
3.2.1.18 alpha(2-3)sialylparagloboside + H2O
-
 Homo sapiens ?
-
 ?
3.2.1.18 alpha-acid glycoprotein + H2O
-
 Homo sapiens ?
-
 ?
3.2.1.18 bovine gland submaxillary gland mucin + H2O
-
 Homo sapiens ?
-
 ?
3.2.1.18 fetuin + H2O
-
 Homo sapiens ?
-
 ?
3.2.1.18 ganglioside GD1a + H2O
-
 Homo sapiens ?
-
 ?
3.2.1.18 ganglioside GD1b + H2O
-
 Homo sapiens ?
-
 ?
3.2.1.18 ganglioside GM3 + H2O
-
 Homo sapiens ?
-
 ?
3.2.1.18 ganglioside GT1b + H2O
-
 Homo sapiens ?
-
 ?
3.2.1.18 transferrin + H2O
-
 Homo sapiens ?
-
 ?
3.2.1.18 vesicular GM3 + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.18 HsNEU2
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.18 4
-
 24 h, 50% loss of activity Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.18 5.6
-
-
 Homo sapiens