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Literature summary extracted from

  • Guo, Q.; Shen, Y.; Zhukovskaya, N.L.; Florian, J.; Tang, W.J.
    Structural and Kinetic Analyses of the Interaction of Anthrax Adenylyl Cyclase Toxin with Reaction Products cAMP and Pyrophosphate (2004), J. Biol. Chem., 279, 29427-29435.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.1 expression in Escherichia coli Bacillus anthracis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.6.1.1 3.2 A resolution Bacillus anthracis

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.1 K372A mutant with 30fold reduced catalytic rate constant and 3fold increased Km value for ATP Bacillus anthracis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.6.1.1 cAMP mutant K372A shows at least 20fold increase in the IC50 value Bacillus anthracis
4.6.1.1 diphosphate no difference between wild type and mutant K372A Bacillus anthracis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6.1.1 0.3
-
 ATP 30°C, pH 7.2, 2 microM free Ca2+ Bacillus anthracis
4.6.1.1 1.3
-
 ATP 30°C, pH 7.2, 0.1 microM free Ca2+ Bacillus anthracis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.6.1.1 Mg2+
-
 Bacillus anthracis

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.1 Bacillus anthracis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.1
-
 Bacillus anthracis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.6.1.1 additional information
-
 Vmax forward reaction 1200/s, pH 7.2, 30°C, 0.1 microM free Ca2+, 147/s pH 7.2, 30°C, 2 microM free Ca2+ Bacillus anthracis
4.6.1.1 additional information
-
 Vmax reverse reaction 2000/s, pH 7.2, 30°C, 0.1 microM free Ca2+, 100-300/s pH 7.2, 30°C, 2 microM free Ca2+ Bacillus anthracis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.1 ATP
-
 Bacillus anthracis cAMP + diphosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.6.1.1 adenylyl cyclase
-
 Bacillus anthracis
4.6.1.1 Edema factor
-
 Bacillus anthracis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.6.1.1 0.06
-
 diphosphate forward reaction, 2 microM free Ca2+, pH 7.2, 30°C Bacillus anthracis
4.6.1.1 0.3
-
 diphosphate forward reaction, 0.1 microM free Ca2+, pH 7.2, 30°C Bacillus anthracis
4.6.1.1 1
-
 diphosphate reverse reaction, 0.1 microM free Ca2+, pH 7.2, 30°C Bacillus anthracis
4.6.1.1 1.2
-
 diphosphate reverse reaction, 2 microM free Ca2+, pH 7.2, 30°C Bacillus anthracis
4.6.1.1 1.3
-
 cAMP forward reaction, 2 microM free Ca2+, pH 7.2, 30°C Bacillus anthracis
4.6.1.1 1.5
-
 cAMP reverse reaction, 0.1 microM free Ca2+, pH 7.2, 30°C Bacillus anthracis
4.6.1.1 2
-
 cAMP reverse reaction, 2 microM free Ca2+, pH 7.2, 30°C Bacillus anthracis
4.6.1.1 3.7
-
 cAMP forward reaction, 0.1 microM free Ca2+, pH 7.2, 30°C Bacillus anthracis