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Literature summary extracted from

  • Dias, F.M.; Vincent, F.; Pell, G.; Prates, J.A.; Centeno, M.S.; Tailford, L.E.; Ferreira, L.M.; Fontes, C.M.; Davies, G.J.; Gilbert, H.J.
    Insights into the molecular determinants of substrate specificity in glycoside hydrolase family 5 revealed by the crystal structure and kinetics of Cellvibrio mixtus mannosidase 5A (2004), J. Biol. Chem., 279, 25517-25526.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.25 expression in Escherichia coli Cellvibrio mixtus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.25 hanging drop vapour diffusion method Cellvibrio mixtus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.25 E330A mutant enzyme displays no activity against mannan or 4-nitrophenyl-beta-mannopyranoside Cellvibrio mixtus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.25 0.000083
-
 4-nitrophenyl-beta-mannopyranose pH 7.0, 37°C, mutant enzyme E215A Cellvibrio mixtus
3.2.1.25 0.1
-
 Mannotriose pH 7.0, 37°C, wild-type enzyme Cellvibrio mixtus
3.2.1.25 0.9
-
 mannobiose pH 7.0, 37°C, wild-type enzyme Cellvibrio mixtus
3.2.1.25 1.6
-
 4-nitrophenyl-beta-D-mannopyranose pH 7.0, 37°C, wild-type enzyme Cellvibrio mixtus
3.2.1.25 6.1
-
 beta-D-mannosyl-1,4-beta-D-glucoside pH 7.0, 37°C, wild-type enzyme Cellvibrio mixtus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.25 Cellvibrio mixtus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.25 recombinant Cellvibrio mixtus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.25 1-galactosyl-mannotriose + H2O
-
 Cellvibrio mixtus ?
-
 ?
3.2.1.25 4-nitrophenyl beta-D-mannopyranoside + H2O
-
 Cellvibrio mixtus 4-nitrophenol + alpha-D-mannose
-
 ?
3.2.1.25 4-O-beta-D-mannopyranosyl-D-mannopyranose + H2O
-
 Cellvibrio mixtus D-mannopyranose
-
 ?
3.2.1.25 beta-D-mannosyl-1,4-beta-D-glucoside + H2O
-
 Cellvibrio mixtus D-mannose + beta-D-glucose
-
 ?
3.2.1.25 mannobiose + H2O
-
 Cellvibrio mixtus 2 D-mannopyranose
-
 ?
3.2.1.25 mannotetraose + H2O
-
 Cellvibrio mixtus ?
-
 ?
3.2.1.25 mannotriose + H2O
-
 Cellvibrio mixtus ?
-
 ?
3.2.1.25 additional information CmMan5A releases beta-1,4-linked mannose substrates exclusively from the nonreducing end Cellvibrio mixtus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.25 CmMan5a
-
 Cellvibrio mixtus
3.2.1.25 mannosidase 5A
-
 Cellvibrio mixtus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.25 0.033
-
 4-nitrophenyl-beta-D-mannopyranose pH 7.0, 37°C, wild-type enzyme Cellvibrio mixtus
3.2.1.25 0.053
-
 beta-D-mannosyl-1,4-beta-D-glucoside pH 7.0, 37°C, wild-type enzyme Cellvibrio mixtus
3.2.1.25 0.1
-
 4-nitrophenyl-beta-mannopyranose pH 7.0, 37°C, mutant enzyme E215A Cellvibrio mixtus
3.2.1.25 2.17
-
 4-O-beta-D-mannopyranosyl-D-mannopyranose pH 7.0, 37°C, wild-type enzyme Cellvibrio mixtus
3.2.1.25 166.7
-
 Mannotriose pH 7.0, 37°C, wild-type enzyme Cellvibrio mixtus