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Literature summary extracted from

  • Browne, G.J.; Finn, S.G.; Proud, C.G.
    Stimulation of the AMP-activated protein kinase leads to activation of eukaryotic elongation factor 2 kinase and to its phosphorylation at a novel site, serine 398 (2004), J. Biol. Chem., 279, 12220-12231.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.20 2-deoxy-D-glucose
-
 Homo sapiens
2.7.11.20 2-deoxy-D-glucose
-
 Rattus norvegicus
2.7.11.20 5-amino-4-imidazolecarboxyamide riboside
-
 Homo sapiens
2.7.11.20 5-amino-4-imidazolecarboxyamide riboside
-
 Rattus norvegicus
2.7.11.20 Calmodulin dependent on Rattus norvegicus
2.7.11.20 Calmodulin completely dependent on Homo sapiens
2.7.11.20 carbonyl cyanide 3-chlorophenylhydrazone
-
 Homo sapiens
2.7.11.20 additional information ATP depletion activates the enzyme Homo sapiens
2.7.11.20 additional information ATP depletion activates the enzyme in cardiomyocytes Rattus norvegicus
2.7.11.20 Rapamycin
-
 Homo sapiens
2.7.11.20 Rapamycin
-
 Rattus norvegicus
2.7.11.31 5-amino-4-imidazolecarboxamide riboside
-
 Homo sapiens
2.7.11.31 cAMP dependent on, stimulates Homo sapiens
2.7.11.31 additional information ATP depletion activates the enzyme Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.20 expression of GST-tagged wild-type enzyme and of a FLAG-tagged mutant enzyme in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.20 S398A site-directed mutagenesis, mutation of a phosphorylation site, no phosphorylation by AMPK, altered regulation by phosphorylation compared to the wild-type enzyme Homo sapiens
2.7.11.20 S78A site-directed mutagenesis, mutation of a phosphorylation site, altered regulation by phosphorylation compared to the wild-type enzyme, but the mutation does not influence AMPK Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.20 Ca2+ completely dependent on, activates Homo sapiens
2.7.11.20 Ca2+ dependent on, activates Rattus norvegicus
2.7.11.20 Mg2+
-
 Homo sapiens
2.7.11.20 Mg2+
-
 Rattus norvegicus
2.7.11.31 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.20 ATP + [elongation factor 2] Rattus norvegicus
-
 ADP + [elongation factor 2]phosphate
-
 ?
2.7.11.20 ATP + [elongation factor 2] Homo sapiens enzyme regulation by de-/phosphorylation, overview ADP + [elongation factor 2]phosphate
-
 ?
2.7.11.31 ATP + eukaryotic elongation factor 2 kinase Homo sapiens phosphorylation at Ser398, the enzyme plays a regulatory role in eEF2 kinase activity, overview ADP + phosphorylated eukaryotic elongation factor 2 kinase
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.20 Homo sapiens
-
-
-
2.7.11.20 Rattus norvegicus
-
-
-
2.7.11.31 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.20 phosphoprotein the enzyme performs autophosphorylation, stimulation of AMP-activated protein kinase, EC 2.7.11.11, by AMP leads to activation of the enzyme and to its phosphorylation at Ser398 in the regulatory domain, other phosphorylation sites of the enzyme are Ser78, Ser359, Ser377, and Ser366 Rattus norvegicus
2.7.11.20 phosphoprotein the enzyme performs autophosphorylation, stimulation of AMP-activated protein kinase, EC 2.7.11.11, by AMP leads to activation of the wild-type and mutant enzyme and to its phosphorylation at Ser398 in the regulatory domain, other phosphorylation sites of the enzyme are Ser78, Ser359, Ser377, and Ser366, the latter is phosphorylated by kinases S6K1 and p90RSK inhibiting the enzyme Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.20 recombinant GST-tagged wild-type and FLAG-tagged mutant enzymes from Escherichia coli Homo sapiens
2.7.11.31 from KB cells by several steps including immunoprecipitation Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.20 cardiac myocyte ventricle Rattus norvegicus
-
2.7.11.20 heart ventricle
-
 Rattus norvegicus
-
2.7.11.20 KB cell oral epidermoid carcinoma cell line Homo sapiens
-
2.7.11.31 KB cell oral epidermoid carcinoma cell line Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.20 ATP + [elongation factor 2]
-
 Homo sapiens ADP + [elongation factor 2]phosphate
-
 ?
2.7.11.20 ATP + [elongation factor 2]
-
 Rattus norvegicus ADP + [elongation factor 2]phosphate
-
 ?
2.7.11.20 ATP + [elongation factor 2] enzyme regulation by de-/phosphorylation, overview Homo sapiens ADP + [elongation factor 2]phosphate
-
 ?
2.7.11.31 ATP + eukaryotic elongation factor 2 kinase phosphorylation at Ser398, the enzyme plays a regulatory role in eEF2 kinase activity, overview Homo sapiens ADP + phosphorylated eukaryotic elongation factor 2 kinase
-
 ?
2.7.11.31 ATP + eukaryotic elongation factor 2 kinase phosphorylation at Ser398 activates the eukaryotic elongation factor 2 kinase, no activity with the substrate mutant S398A Homo sapiens ADP + phosphorylated eukaryotic elongation factor 2 kinase
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.20 More recombinant GST-tagged enzyme peptide mapping Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.11.20 eEF2 kinase
-
 Homo sapiens
2.7.11.20 eEF2 kinase
-
 Rattus norvegicus
2.7.11.20 eukaryotic elongation factor 2 kinase
-
 Homo sapiens
2.7.11.20 eukaryotic elongation factor 2 kinase
-
 Rattus norvegicus
2.7.11.31 AMP-activated protein kinase
-
 Homo sapiens
2.7.11.31 AMPK
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.20 30
-
 assay at Homo sapiens
2.7.11.20 30
-
 assay at Rattus norvegicus
2.7.11.31 30
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.20 7
-
 assay at Homo sapiens
2.7.11.20 7
-
 assay at Rattus norvegicus
2.7.11.31 7
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.20 ATP
-
 Homo sapiens
2.7.11.20 ATP
-
 Rattus norvegicus
2.7.11.31 ATP
-
 Homo sapiens