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Literature summary extracted from

  • Tsay, J.T.; Oh, W.; Larson, T.J.; Jackowski, S.; Rock, C.O.
    Isolation and characterization of the beta-ketoacyl-acyl carrier protein synthase III gene (fabH) from Escherichia coli K-12 (1992), J. Biol. Chem., 267, 6807-6814.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.180 gene fabH, gene is located on the chromosome, DNA and amino acid sequence determination and analysis, functional complementation of enzyme-defective fabH1 mutant strain CDM5, expression in strain JT2038 alters the endogenous fatty acid composition Escherichia coli K-12

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.180 33517
-
 x * 33517, DNA sequence calculation, x * 34800, recombinant enzyme, SDS-PAGE Escherichia coli K-12
2.3.1.180 34800
-
 x * 33517, DNA sequence calculation, x * 34800, recombinant enzyme, SDS-PAGE Escherichia coli K-12

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein] Escherichia coli K-12
-
 acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.180 Escherichia coli K-12 P0A6R0 gene fabH
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.180 recombinant enzyme from strain JT2038 by ammonium sulfate fractionation, gel filtration, and affinity chromatography Escherichia coli K-12

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.180 acetyl-CoA + a malonyl-[acyl-carrier protein] = an acetoacetyl-[acyl-carrier protein] + CoA + CO2 the active site is formed by Ala-Cys-Ala Escherichia coli K-12
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.180 0.00013
-
 acetyl transacetylase activity, recombinant strain JT2038 Escherichia coli K-12
2.3.1.180 0.024
-
 acetoacetyl-ACP synthase activity, recombinant strain JT2038 Escherichia coli K-12

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein]
-
 Escherichia coli K-12 acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 r
2.3.1.180 acetyl-CoA + [acyl-carrier protein] the enzyme also shows acetyltransferase activity Escherichia coli K-12 acetyl-[acyl-carrier protein] + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.180 ? x * 33517, DNA sequence calculation, x * 34800, recombinant enzyme, SDS-PAGE Escherichia coli K-12

Synonyms

EC Number Synonyms Comment Organism
2.3.1.180 acetoacetyl-ACP synthase
-
 Escherichia coli K-12
2.3.1.180 acetyl-CoA:ACP transacylase
-
 Escherichia coli K-12
2.3.1.180 ACP synthase III
-
 Escherichia coli K-12
2.3.1.180 beta-ketoacyl-acyl carrier protein synthase III
-
 Escherichia coli K-12

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.180 37
-
 assay at Escherichia coli K-12

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.180 7
-
 assay at Escherichia coli K-12

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.3.1.180 Escherichia coli K-12 DNA sequence calculation
-
 4.85