EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.2.2.7 | EDTA | 0.1 mM, 104% increase in activity | Bacteroides stercoris | |
4.2.2.7 | iodoacetic acid | 0.1 mM, 53% increase in activity | Bacteroides stercoris |
EC Number | General Stability | Organism |
---|---|---|
4.2.2.7 | enzyme is stabilized by dithiothreitol | Bacteroides stercoris |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.2.7 | carbodiimide | 0.1 mM, 27% inhibition | Bacteroides stercoris | |
4.2.2.7 | Cd2+ | 0.1 mM, 22% inhibition | Bacteroides stercoris | |
4.2.2.7 | Mg2+ | 0.1 mM, 17% inhibition | Bacteroides stercoris | |
4.2.2.7 | N-tosyl-L-lysine-chloromethyl ketone | 0.1 mM, 84% inhibition | Bacteroides stercoris | |
4.2.2.7 | p-chloromercuriphenyl sulfonic acid | 0.1 mM, complete inhibition | Bacteroides stercoris |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.2.7 | 0.013 | - |
heparin | pH 7.0, 45°C, heparin from porcine mucosa | Bacteroides stercoris | |
4.2.2.7 | 0.21 | - |
heparan sulfate | pH 7.0, 45°C, heparin from porcine mucosa | Bacteroides stercoris |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.2.7 | Co2+ | 0.1 mM, 123% increase in activity | Bacteroides stercoris | |
4.2.2.7 | Cu2+ | 0.1 mM, 14% increase in activity | Bacteroides stercoris | |
4.2.2.7 | NaCl | 500 mM, required for maximal activity | Bacteroides stercoris |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.2.7 | 48000 | - |
x * 48000, SDS-PAGE | Bacteroides stercoris |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.2.7 | Bacteroides stercoris | - |
- |
- |
4.2.2.7 | Bacteroides stercoris HJ-15 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.2.7 | - |
Bacteroides stercoris |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.2.2.7 | intestine | - |
Bacteroides stercoris | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.2.7 | 2-O-desulfated heparin | activity is 26% of the activity with heparin | Bacteroides stercoris | ? | - |
? | |
4.2.2.7 | 2-O-desulfated heparin | activity is 26% of the activity with heparin | Bacteroides stercoris HJ-15 | ? | - |
? | |
4.2.2.7 | heparan sulfate | enzyme prefers heparin to heparan sulfate. Activity with heparan sulfate from porcine mucosa is 17% of the activity with heparin. Activity with heparan sulfate from bovine kidney is 15% of the activity with heparin | Bacteroides stercoris | ? | - |
? | |
4.2.2.7 | heparan sulfate | enzyme prefers heparin to heparan sulfate. Activity with heparan sulfate from porcine mucosa is 17% of the activity with heparin. Activity with heparan sulfate from bovine kidney is 15% of the activity with heparin | Bacteroides stercoris HJ-15 | ? | - |
? | |
4.2.2.7 | heparin | enzyme prefers heparin to heparan sulfate. The enzyme acts preferentially on the trisulfated sequence ->4-alpha-D-GlcNS6S(1->4)-alpha-L-IdoA2S1-> comprising heparin affording the unsaturated trisulfated disaccharid (DELTAUA2S-GlcNS6S) | Bacteroides stercoris | ? | - |
? | |
4.2.2.7 | heparin | enzyme prefers heparin to heparan sulfate. The enzyme acts preferentially on the trisulfated sequence ->4-alpha-D-GlcNS6S(1->4)-alpha-L-IdoA2S1-> comprising heparin affording the unsaturated trisulfated disaccharid (DELTAUA2S-GlcNS6S) | Bacteroides stercoris HJ-15 | ? | - |
? | |
4.2.2.7 | additional information | no activity with acharan sulfate, chondroitin sulfate and N-desulfated heparin | Bacteroides stercoris | ? | - |
? | |
4.2.2.7 | additional information | no activity with acharan sulfate, chondroitin sulfate and N-desulfated heparin | Bacteroides stercoris HJ-15 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.2.7 | ? | x * 48000, SDS-PAGE | Bacteroides stercoris |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.2.7 | heparin lyase I | - |
Bacteroides stercoris |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.2.7 | 50 | - |
- |
Bacteroides stercoris |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.2.7 | 30 | 56 | 30°C: about 40% of maximal activity, 56°C: about 650% of maximal activity | Bacteroides stercoris |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.2.7 | 7 | - |
- |
Bacteroides stercoris |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
4.2.2.7 | Bacteroides stercoris | isoelectric focusing | - |
9 |