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Literature summary extracted from

  • Kaulpiboon, J.; Pongsawasdi, P.
    Expression of cyclodextrinase gene from Paenibacillus sp. A11 in Escherichia coli and characterization of the purified cyclodextrinase (2004), J. Biochem. Mol. Biol., 37, 408-415.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.54 dithiothreitol recombinant enzyme Paenibacillus sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.54 expression by using the pUC 18 vector in Escherichia coli JM 109 Paenibacillus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.54 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide 5 mM, complete inhibition Paenibacillus sp.
3.2.1.54 AgNO3 10 mM, complete inhibition Paenibacillus sp.
3.2.1.54 BaCl2 10 mM, 12% inhibition Paenibacillus sp.
3.2.1.54 diethyldicarbonate 1 mM, complete inhibition, recombinant enzyme Paenibacillus sp.
3.2.1.54 HgCl2 10 mM, complete inhibition Paenibacillus sp.
3.2.1.54 iodoacetamide 5 mM, 29% inhibition Paenibacillus sp.
3.2.1.54 MgCl2 10 mM, 20% inhibition Paenibacillus sp.
3.2.1.54 N-bromosuccinimide 0.1 mM, 95% inhibition, recombinant enzyme Paenibacillus sp.
3.2.1.54 Phenylglyoxal 5 mM, 38% inhibition Paenibacillus sp.
3.2.1.54 ZnCl2 10 mM, 63% inhibition Paenibacillus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.54 2.5
-
 beta-cyclodextrin
-
 Paenibacillus sp.
3.2.1.54 2.9
-
 gamma-cyclodextrin
-
 Paenibacillus sp.
3.2.1.54 5
-
 alpha-cyclodextrin
-
 Paenibacillus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.54 cytoplasm recombinant enzyme is produced in Escherichia coli primarily in Paenibacillus sp. 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.54 80000
-
 gel filtration Paenibacillus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.54 Paenibacillus sp.
-
-
-
3.2.1.54 Paenibacillus sp. A11
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.54 recombinant Paenibacillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.54 alpha-cyclodextrin + H2O 57% of the activity with beta-cyclodextrin Paenibacillus sp. D-glucose + maltose + less amounts of maltotriose and maltotetraose ?
3.2.1.54 beta-cyclodextrin + H2O
-
 Paenibacillus sp. D-glucose + maltose + less amounts of maltotriose and maltotetraose ?
3.2.1.54 gamma-cyclodextrin + H2O 72% of the activity with beta-cyclodextrin Paenibacillus sp. D-glucose + maltose + less amounts of maltotriose and maltotetraose ?
3.2.1.54 maltoheptaose + H2O 43% of the activity with beta-cyclodextrin Paenibacillus sp. D-glucose + maltose + ?
-
 ?
3.2.1.54 maltoheptaose + H2O 43% of the activity with beta-cyclodextrin Paenibacillus sp. A11 D-glucose + maltose + ?
-
 ?
3.2.1.54 maltohexaose + H2O 35% of the activity with beta-cyclodextrin Paenibacillus sp. D-glucose + maltose + ?
-
 ?
3.2.1.54 maltohexaose + H2O 35% of the activity with beta-cyclodextrin Paenibacillus sp. A11 D-glucose + maltose + ?
-
 ?
3.2.1.54 maltopentaose + H2O 22% of the activity with beta-cyclodextrin Paenibacillus sp. D-glucose + maltose + ?
-
 ?
3.2.1.54 maltopentaose + H2O 22% of the activity with beta-cyclodextrin Paenibacillus sp. A11 D-glucose + maltose + ?
-
 ?
3.2.1.54 maltotetraose + H2O 14% of the activity with beta-cyclodextrin Paenibacillus sp. 2 D-glucose + maltose
-
 ?
3.2.1.54 maltotetraose + H2O 14% of the activity with beta-cyclodextrin Paenibacillus sp. A11 2 D-glucose + maltose
-
 ?
3.2.1.54 maltotriose + H2O 11% of the activity with beta-cyclodextrin Paenibacillus sp. maltose + D-glucose
-
 ?
3.2.1.54 maltotriose + H2O 11% of the activity with beta-cyclodextrin Paenibacillus sp. A11 maltose + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.54 monomer 1 * 80000, SDS-PAGE Paenibacillus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.54 40
-
-
 Paenibacillus sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.54 25
-
 alpha-cyclodextrin
-
 Paenibacillus sp.
3.2.1.54 26.7
-
 gamma-cyclodextrin
-
 Paenibacillus sp.
3.2.1.54 36.7
-
 beta-cyclodextrin
-
 Paenibacillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.54 7
-
-
 Paenibacillus sp.