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Literature summary extracted from

  • Kim, H.J.; Tamanoue, Y.; Jeohn, G.H.; Iwamatsu, A.; Yokota, A.; Kim, Y.T.; Takahashi, T.; Takahashi, K.
    Purification and characterization of an extracellular metalloprotease from Pseudomonas fluorescens (1997), J. Biochem., 121, 82-88.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.24.40 t-butyloxycarbonyl-Arg-Val-Arg-Arg-4-methylcoumaryl-7-amide
-
 Pseudomonas fluorescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.40 1,10-phenanthroline 10 mM, strong inhibition at pH 5.5 and pH 8.0 with and without addition of 1 mM Co2+ Pseudomonas fluorescens
3.4.24.40 Bovine pancreatic trypsin inhibitor 0.01 mM, 5% inhibition at pH 5.5 Pseudomonas fluorescens
3.4.24.40 chymostatin 0.1 mM, 18% inhibition at pH 8.0 Pseudomonas fluorescens
3.4.24.40 diisopropylfluorophosphate 1 mM, weak inhibition at pH 5.5 and pH 8.0 with and without addition of 1 mM Co2+ Pseudomonas fluorescens
3.4.24.40 EDTA 10 mM, almost complete inactivation at pH 5.5 and pH 8.0 with and without addition of 1 mM Co2+ Pseudomonas fluorescens
3.4.24.40 Elastatinal 0.1 mM, 11% inhibition at pH 8.0 and without 1 mM Co2+ addition Pseudomonas fluorescens
3.4.24.40 L-trans-epoxysuccinyl-leucyl-amido-(4-guanido)butane 0.1 mM, 26% inhibition at pH 8.0 and 1 mM Co2+ addition, 26% inhibition at pH 5.5 without addition of 1 mM Co2+ Pseudomonas fluorescens
3.4.24.40 leupeptin 0.1 mM, 10% inhibition at pH 8.0 and 1 mM Co2+ addition, 7% inhibition at pH 8.0 without 1 mM Co2+ addition Pseudomonas fluorescens
3.4.24.40 N-ethylmaleimide 0.1 mM, 14% inhibition at pH 8.0 and 1 mM Co2+ addition Pseudomonas fluorescens
3.4.24.40 N-tosyl-L-phenylalanine chloromethyl ketone 0.1 mM, 18% inhibition at pH 8.0 Pseudomonas fluorescens
3.4.24.40 phenyl-methanesulfonyl fluoride 1 mM, 14-17% inhibition at pH 5.5 and pH 8.0 with and without addition of 1 mM Co2+ Pseudomonas fluorescens
3.4.24.40 phosphoramidon 0.1 mM, no inhibition at pH 8.0 and 1 mM Co2+ addition, 25% inhibition at pH 8.0 and 5.5 without addition of 1 mM Co2+ Pseudomonas fluorescens
3.4.24.40 Soybean trypsin inhibitor 0.01 mM, 9% inhibition at pH 5.5 Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.40 0.015
-
 t-butyloxycarbonyl-Arg-Val-Arg-Arg-4-methylcoumaryl-7-amide without Co2+, pH 8.0 Pseudomonas fluorescens
3.4.24.40 0.016
-
 t-butyloxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide without Co2+, pH 8.0 Pseudomonas fluorescens
3.4.24.40 0.023
-
 t-butyloxycarbonyl-Arg-Val-Arg-Arg-4-methylcoumaryl-7-amide 1 mM Co2+, pH 5.5 Pseudomonas fluorescens
3.4.24.40 0.023
-
 t-butyloxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide 1 mM Co2+, pH 8.0 Pseudomonas fluorescens
3.4.24.40 0.023
-
 t-butyloxycarbonyl-Arg-Val-Arg-Arg-4-methylcoumaryl-7-amide without Co2+, pH 5.5 Pseudomonas fluorescens
3.4.24.40 0.035
-
 t-butyloxycarbonyl-Arg-Val-Arg-Arg-4-methylcoumaryl-7-amide 1 mM Co2+, pH 8.0 Pseudomonas fluorescens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.40 extracellular
-
 Pseudomonas fluorescens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.40 Co2+
-
 Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.40 46000
-
 gel filtration Pseudomonas fluorescens
3.4.24.40 47000
-
 SDS-PAGE Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.40 Pseudomonas fluorescens
-
 KT1
-
3.4.24.40 Pseudomonas fluorescens KT1
-
 KT1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.40 dialysis against 10 mM N-methyldiethanolamine, DE-52 gel filtration, chelating Sepharose 6B gel filtration, dialysis against 0.02M triethanolamine-HCl buffer Pseudomonas fluorescens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.40 3.98
-
 7-amino-4-methyl-coumarin, 37°C, pH 8.0 Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.40 t-butyloxycarbonyl-Ala-Gly-Pro-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas fluorescens ?
-
 ?
3.4.24.40 t-butyloxycarbonyl-Ala-Gly-Pro-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas fluorescens KT1 ?
-
 ?
3.4.24.40 t-butyloxycarbonyl-Arg-Val-Arg-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas fluorescens ?
-
 ?
3.4.24.40 t-butyloxycarbonyl-Arg-Val-Arg-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas fluorescens KT1 ?
-
 ?
3.4.24.40 t-butyloxycarbonyl-Gln-Ala-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas fluorescens ?
-
 ?
3.4.24.40 t-butyloxycarbonyl-Gln-Arg-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas fluorescens ?
-
 ?
3.4.24.40 t-butyloxycarbonyl-Gln-Arg-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas fluorescens KT1 ?
-
 ?
3.4.24.40 t-butyloxycarbonyl-Glu-Lys-Lys-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas fluorescens ?
-
 ?
3.4.24.40 t-butyloxycarbonyl-Gly-Arg-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas fluorescens ?
-
 ?
3.4.24.40 t-butyloxycarbonyl-Gly-Lys-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas fluorescens ?
-
 ?
3.4.24.40 t-butyloxycarbonyl-Leu-Arg-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas fluorescens ?
-
 ?
3.4.24.40 t-butyloxycarbonyl-Leu-Lys-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas fluorescens ?
-
 ?
3.4.24.40 t-butyloxycarbonyl-Leu-Ser-Thr-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas fluorescens ?
-
 ?
3.4.24.40 t-butyloxycarbonyl-Leu-Ser-Thr-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas fluorescens KT1 ?
-
 ?
3.4.24.40 t-butyloxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas fluorescens ?
-
 ?
3.4.24.40 t-butyloxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas fluorescens KT1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.40 monomer
-
 Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.40 5.5
-
 with substrate t-butyloxycarbonyl-Arg-Val-Arg-Arg-4-methylcoumaryl-7-amide in absence and presence of Co2+ Pseudomonas fluorescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.40 6 8 in absence and presence of Co2+ Pseudomonas fluorescens
3.4.24.40 8 9 with substrate t-butyloxycarbonyl-Arg-Val-Arg-Arg-4-methylcoumaryl-7-amide in the presence of Co2+ Pseudomonas fluorescens