Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Abe, T.; Kobayashi, T.; Saito, T.
    Properties of a novel intracellular poly(3-hydroxybutyrate) depolymerase with high specific activity (PhaZd) in Wautersia eutropha H16 (2005), J. Bacteriol., 187, 6982-6990.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.75 EDTA at 1 mM 130% activity, at 10 mM 140% activity Cupriavidus necator
3.1.1.75 Triton X-100 at 0.001% (w/v) 120% activity Cupriavidus necator

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.75 expression in Escherichia coli Cupriavidus necator

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.75 CaCl2 at 10 mM 39% inhibition Cupriavidus necator
3.1.1.75 diisopropylfluorophosphate at 10 microM 53% inhibition, at 100 microM total loss of activity Cupriavidus necator
3.1.1.75 dithiothreitol at 0.1 mM 86% inhibition, complete inhibition at 1 mM Cupriavidus necator
3.1.1.75 MgCl2 slight inhibition 1 mM, 57% inhibition at 10 mM Cupriavidus necator
3.1.1.75 additional information no inhibition by phenylmethylsulfonyl fluoride Cupriavidus necator
3.1.1.75 p-(chloromercuri)benzoic acid no inhibition below 1 mM Cupriavidus necator
3.1.1.75 SDS at 0.01% (w/v) 28% inhibition, at 0.1% complete inhibition Cupriavidus necator
3.1.1.75 Triton X-100 at 0.1% 57% inhibition, at 1% 64% inhibition Cupriavidus necator

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.75 intracellular partially at poly(3-hydroxybutyrate) bodies and in the cytosol Cupriavidus necator 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.75 39168
-
 1 * 39168, SDS-PAGE Cupriavidus necator
3.1.1.75 39170
-
 deduced from sequence, SDS-PAGE Cupriavidus necator

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O Cupriavidus necator specific for amorphous poly(3-hydroxybutyrate) granules poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 specific for amorphous poly(3-hydroxybutyrate) granules poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.75 Cupriavidus necator
-
-
-
3.1.1.75 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.75 His-tag fusion, Ni-affinity chromatography Cupriavidus necator

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.75 cell culture
-
 Cupriavidus necator
-

Storage Stability

EC Number Storage Stability Organism
3.1.1.75 -20°C, 20 mM Tris-HCl pH 8.0 50% glycerol, sable for more than 3 months Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.75 p-nitrophenyl acetate + H2O
-
 Cupriavidus necator p-nitrophenol + acetate
-
 ?
3.1.1.75 p-nitrophenyl acetate + H2O
-
 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 p-nitrophenol + acetate
-
 ?
3.1.1.75 p-nitrophenyl butyrate + H2O
-
 Cupriavidus necator p-nitrophenol + butyrate
-
 ?
3.1.1.75 p-nitrophenyl butyrate + H2O
-
 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 p-nitrophenol + butyrate
-
 ?
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O specific for amorphous poly(3-hydroxybutyrate) granules Cupriavidus necator poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O specific for amorphous poly(3-hydroxybutyrate) granules, weak activity on 3-hydroxybutyrate dimers and trimers, no activity on tetramers or pentamers Cupriavidus necator poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O specific for amorphous poly(3-hydroxybutyrate) granules Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O specific for amorphous poly(3-hydroxybutyrate) granules, weak activity on 3-hydroxybutyrate dimers and trimers, no activity on tetramers or pentamers Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.75 monomer 1 * 39168, SDS-PAGE Cupriavidus necator

Synonyms

EC Number Synonyms Comment Organism
3.1.1.75 PhaZd
-
 Cupriavidus necator
3.1.1.75 poly(3-hydroxybutyrate) depolymerase
-
 Cupriavidus necator

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.75 20 30
-
 Cupriavidus necator

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.75 30
-
 stable for 10 min, at 40°C 80% activity is lost after 10 min, above 50°C all activity is lost Cupriavidus necator

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.75 8.5 9 in Tris-HCl buffer Cupriavidus necator