Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Li, R.; Potters, M.B.; Shi, L.; Kennelly, P.J.
    The protein phosphatases of Synechocystis sp. strain PCC 6803: open reading frames sll1033 and sll1387 encode enzymes that exhibit both protein-serine and protein-tyrosine phosphatase activity in vitro (2005), J. Bacteriol., 187, 5877-5884.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.16 genes sll1033 and sll1387 encoding SynPPM3 and SynPPP1, DNA and amino acid sequence determination and analysis, expression analysis, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Synechocystis sp.

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.16 D415A site-directed mutagenesis of SynPPM3, the mutant shows reduced activity compared to the wild-type enzyme, the loss of activity differs in dependence of the substrate Synechocystis sp.
3.1.3.16 D415N site-directed mutagenesis of SynPPM3, the mutant shows reduced activity compared to the wild-type enzyme, the loss of activity differs in dependence of the substrate Synechocystis sp.
3.1.3.16 D444N site-directed mutagenesis of SynPPM3, the mutant shows reduced activity compared to the wild-type enzyme, the loss of activity differs in dependence of the substrate Synechocystis sp.
3.1.3.16 D604N site-directed mutagenesis of SynPPM3, the mutant shows reduced activity compared to the wild-type enzyme, the loss of activity differs in dependence of the substrate Synechocystis sp.
3.1.3.16 D608N site-directed mutagenesis of SynPPM3, the mutant shows reduced activity compared to the wild-type enzyme, the loss of activity differs in dependence of the substrate, the activity with phosphotyrosine-RCML is highly increased compared to the wild-type enzyme Synechocystis sp.
3.1.3.16 D648N site-directed mutagenesis of SynPPM3, the mutant shows reduced activity compared to the wild-type enzyme, the loss of activity differs in dependence of the substrate Synechocystis sp.
3.1.3.16 E414Q site-directed mutagenesis of SynPPM3, the mutant shows reduced activity compared to the wild-type enzyme, the loss of activity differs in dependence of the substrate, the activity with 4-nitropehnyl phosphate is similar to the wild-type enzyme Synechocystis sp.
3.1.3.16 E618Q site-directed mutagenesis of SynPPM3, the mutant shows reduced activity compared to the wild-type enzyme, the loss of activity differs in dependence of the substrate Synechocystis sp.
3.1.3.48 D415A isoform SynPPM3, no activity with 4-nitrophenyl phosphate, reduction in phosphoserine- and phosphotyrosine-activity against casein and carboxyamidomethylated and maleylated lysozyme Synechocystis sp.
3.1.3.48 D415N isoform SynPPM3, 5% activity with 4-nitrophenyl phosphate compared to wild-type, 50% reduction in phosphotyrosine-activity against carboxyamidomethylated and maleylated lysozyme Synechocystis sp.
3.1.3.48 D444N isoform SynPPM3, no activity with 4-nitrophenyl phosphate, reduction in phosphoserine- and phosphotyrosine-activity against casein and carboxyamidomethylated and maleylated lysozyme Synechocystis sp.
3.1.3.48 D604N isoform SynPPM3, no activity Synechocystis sp.
3.1.3.48 D608N isoform SynPPM3, 50% activity with 4-nitrophenyl phosphate compared to wild-type, 4fold increase in phosphotyrosine-activity against carboxyamidomethylated and maleylated lysozyme Synechocystis sp.
3.1.3.48 D648N isoform SynPPM3, 5% activity with 4-nitrophenyl phosphate compared to wild-type, large reduction in phosphoserine- and phosphotyrosine-activity against casein and carboxyamidomethylated and maleylated lysozyme Synechocystis sp.
3.1.3.48 E414Q isoform SynPPM3, similar activity with 4-nitrophenyl phosphate as wild-type, but 50% reduction in phosphoserine- and phosphotyrosine-activity against casein and carboxyamidomethylated and maleylated lysozyme Synechocystis sp.
3.1.3.48 E618Q isoform SynPPM3, 87% of activity with 4-nitrophenyl phosphate compared to wild-type, reduction in phosphoserine- and phosphotyrosine-activity against casein and carboxyamidomethylated and maleylated lysozyme Synechocystis sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.16 EDTA
-
 Synechocystis sp.
3.1.3.48 NaF moderate inhibition, both SynPPP1 and SYnPPM3 Synechocystis sp.
3.1.3.48 o-vanadate both SynPPP1 and SYnPPM3 Synechocystis sp.
3.1.3.48 okadaic acid both SynPPP1 and SYnPPM3 Synechocystis sp.
3.1.3.48 Sodium diphosphate both isoforms, inhibition by millimolar concentrations Synechocystis sp.
3.1.3.48 Tartrate both SynPPP1 and SYnPPM3 Synechocystis sp.
3.1.3.48 tetramisole both SynPPP1 and SYnPPM3 Synechocystis sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.16 0.00044
-
 phosphoserine-casein pH 7.5, 37°, recombinant SynPPM3 Synechocystis sp.
3.1.3.16 0.00045
-
 phosphoserine-myelin basic protein pH 7.5, 37°, recombinant SynPPM3 Synechocystis sp.
3.1.3.16 0.0017
-
 phosphoserine-RCML pH 7.5, 37°, recombinant SynPPP1 Synechocystis sp.
3.1.3.16 0.0063
-
 phosphoserine-myelin basic protein pH 7.5, 37°, recombinant SynPPM3 Synechocystis sp.
3.1.3.16 0.022
-
 phosphoserine-myelin basic protein pH 7.5, 37°, recombinant SynPPP1 Synechocystis sp.
3.1.3.16 12.7
-
 4-nitrophenyl phosphate pH 7.5, 37°, recombinant SynPPM3 Synechocystis sp.
3.1.3.48 0.00044
-
 casein isoform SynPPM3, activity against phosphoserine residues, pH 7.5, 37°C Synechocystis sp.
3.1.3.48 0.00045
-
 carboxyamidomethylated and maleylated lysozyme isoform SynPPM3, activity against phosphotyrosine residues, pH 7.5, 37°C Synechocystis sp.
3.1.3.48 0.0017
-
 carboxyamidomethylated and maleylated lysozyme isoform SynPPP1, activity against phosphoserine residues, pH 7.5, 37°C Synechocystis sp.
3.1.3.48 0.0063
-
 myelin basic protein isoform SynPPM3, activity against phosphoserine residues, pH 7.5, 37°C Synechocystis sp.
3.1.3.48 0.022
-
 myelin basic protein isoform SynPPP1, activity against phosphoserine residues, pH 7.5, 37°C Synechocystis sp.
3.1.3.48 12.7
-
 4-nitrophenyl phosphate isoform SynPPM3, pH 7.5, 37°C Synechocystis sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.16 Mg2+
-
 Synechocystis sp.
3.1.3.16 Mn2+ required Synechocystis sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.16 Synechocystis sp.
-
 strain PCC 6803, genes sll1033 and sll1387 encoding SynPPM3 and SynPPP1
-
3.1.3.48 Synechocystis sp.
-
 strain PCC 6803, isoforms SynPPP1 and SynPPM3 both acting on phosphotyrosine- as well as phosphoserine-containing proteins
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.16 recombinant His-tagged SynPPM3 and SynPPP1 and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Synechocystis sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.16 [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate the enzymes of Synechocystis sp. also perform the protein tyrosine phosphatase reaction, EC 3.1.3.48 Synechocystis sp.
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.16 additional information
-
 relative activities of mutant SynPPM3 compared to wild-type SynPPM3 Synechocystis sp.
3.1.3.16 0.0001
-
 purified recombinant SynPPM3, substrate phosphotyrosine-casein Synechocystis sp.
3.1.3.16 0.0007
-
 purified recombinant SynPPM3, substrate phosphotyrosine-RCML Synechocystis sp.
3.1.3.16 0.0017
-
 purified recombinant SynPPM3, substrate phosphoserine-RCML Synechocystis sp.
3.1.3.16 0.0017
-
 purified recombinant SynPPP1, substrate phosphoserine-myelin basic protein Synechocystis sp.
3.1.3.16 0.0029
-
 purified recombinant SynPPP1, substrate phosphoserine-myelin basic protein Synechocystis sp.
3.1.3.16 0.0034
-
 purified recombinant SynPPP1, substrate phosphoserine-casein Synechocystis sp.
3.1.3.16 0.0044
-
 purified recombinant SynPPP1, substrate phosphotyrosine-casein Synechocystis sp.
3.1.3.16 0.0053
-
 purified recombinant SynPPM3, substrate phosphoserine-casein Synechocystis sp.
3.1.3.16 0.0055
-
 purified recombinant SynPPP1, substrate phosphotyrosine-RCML Synechocystis sp.
3.1.3.16 0.0121
-
 purified recombinant SynPPP1, substrate phosphoserine-RCML Synechocystis sp.
3.1.3.16 0.0178
-
 purified recombinant SynPPM3, substrate phosphoserine-myelin basic protein Synechocystis sp.
3.1.3.16 0.066
-
 purified recombinant SynPPM3, substrate 4-nitrophenyl phosphate Synechocystis sp.
3.1.3.16 0.084
-
 purified recombinant SynPPP1, substrate 4-nitrophenyl phosphate Synechocystis sp.
3.1.3.48 0.066
-
 isoform SynPPM3, pH 7.5, 37°C Synechocystis sp.
3.1.3.48 0.084
-
 isoform SynPPP1, pH 7.5, 37°C Synechocystis sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.16 4-nitrophenyl phosphate + H2O
-
 Synechocystis sp. 4-nitrophenol + phosphate
-
 ?
3.1.3.16 phosphoserine-casein + H2O
-
 Synechocystis sp. casein + phosphate
-
 ?
3.1.3.16 phosphoserine-myelin basic protein + H2O
-
 Synechocystis sp. myelin basic protein + phosphate
-
 ?
3.1.3.16 phosphoserine-RCML + H2O i.e. reduced carboxyamidomethylated and maleylated lysozyme containing phosphoserine Synechocystis sp. RCML + phosphate
-
 ?
3.1.3.16 phosphotyrosine-casein + H2O
-
 Synechocystis sp. casein + phosphate
-
 ?
3.1.3.16 phosphotyrosine-myelin basic protein + H2O
-
 Synechocystis sp. myelin basic protein + phosphate
-
 ?
3.1.3.16 phosphotyrosine-RCML + H2O i.e. reduced carboxyamidomethylated and maleylated lysozyme containing phosphotyrosine Synechocystis sp. RCML + phosphate
-
 ?
3.1.3.48 4-nitrophenyl phosphate + H2O
-
 Synechocystis sp. 4-nitrophenol + phosphate
-
 ?
3.1.3.48 carboxyamidomethylated and maleylated lysozyme + H2O
-
 Synechocystis sp. ?
-
 ?
3.1.3.48 casein + H2O
-
 Synechocystis sp. ?
-
 ?
3.1.3.48 myelin basic protein + H2O
-
 Synechocystis sp. ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.16 More cf. EC 3.1.3.48 Synechocystis sp.
3.1.3.16 protein sewrine/threonine phosphatase
-
 Synechocystis sp.
3.1.3.16 SynPPM3
-
 Synechocystis sp.
3.1.3.16 SynPPP1
-
 Synechocystis sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.16 37
-
 assay at Synechocystis sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.16 7.5
-
 assay at Synechocystis sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.48 6.5 8.5 isoform SynPPM3 Synechocystis sp.
3.1.3.48 7.5 8.4 isoform SynPPP1 Synechocystis sp.