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Literature summary extracted from

  • Xu, H.; Zhang, Y.; Guo, X.; Ren, S.; Staempfli, A.A.; Chiao, J.; Jiang, W.; Zhao, G.
    Isoleucine biosynthesis in Leptospira interrogans serotype lai strain 56601 proceeds via a threonine-independent pathway (2004), J. Bacteriol., 186, 5400-5409.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.21 expression in Escherichia coli Leptospira interrogans
2.3.3.21 gene cimA, DNA and amino acid sequence determination and analysis, expression as His6-tagged enzyme in Escherichia coli Leptospira interrogans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.21 isoleucine
-
 Leptospira interrogans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.21 0.04
-
 pyruvate recombinant enzyme, pH 8.0, 37°C Leptospira interrogans
2.3.3.21 0.043
-
 pyruvate
-
 Leptospira interrogans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.21 60000
-
 x * 60000, recombinant His6-tagged enzyme, SDS-PAGE Leptospira interrogans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.21 acetyl-CoA + pyruvate + H2O Leptospira interrogans the enzyme is involved in the threonine-independent biosynthesis of isoleucine via an alternative beta-methyl-D-malate pathway, the expression of cimA is transcriptionally regulated by isoleucine CoA + (R)-citramalate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.21 Leptospira interrogans
-
 serotype lai strain 56601
-
2.3.3.21 Leptospira interrogans
-
 serotype lai strain 56601, gene cimA or LA2350
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.21
-
 Leptospira interrogans
2.3.3.21 recombinant His6-tagged enzyme 14.3fold from Escherichia coli by nickel affinity chromatography to homogeneity Leptospira interrogans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.21 0.174
-
 crude extract of Escherichia coli recombinantly expressing CimA Leptospira interrogans
2.3.3.21 2.5
-
-
 Leptospira interrogans
2.3.3.21 2.53
-
 purified recombinant His6-tagged enzyme Leptospira interrogans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.21 acetyl-CoA + pyruvate the enzyme is strictly specific for pyruvate as keto acid substrate Leptospira interrogans (3S)-citramalyl-CoA
-
 ?
2.3.3.21 acetyl-CoA + pyruvate + H2O the enzyme is involved in the threonine-independent biosynthesis of isoleucine via an alternative beta-methyl-D-malate pathway, the expression of cimA is transcriptionally regulated by isoleucine Leptospira interrogans CoA + (R)-citramalate
-
 ?
2.3.3.21 acetyl-CoA + pyruvate + H2O the enzyme is strictly specific for pyruvate as the keto acid substrate Leptospira interrogans CoA + (R)-citramalate
-
 ?
2.3.3.21 additional information LA2350 is a citramalate synthase without detectable alpha-isopropylmalate synthase activity Leptospira interrogans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.21 ? x * 60000, recombinant His6-tagged enzyme, SDS-PAGE Leptospira interrogans

Synonyms

EC Number Synonyms Comment Organism
2.3.3.21 CimA
-
 Leptospira interrogans
2.3.3.21 citramalate synthase
-
 Leptospira interrogans
2.3.3.21 LA2350
-
 Leptospira interrogans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.21 37
-
-
 Leptospira interrogans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.3.21 additional information
-
 temperature profile, overview Leptospira interrogans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.3.21
-
 40 80% of maximal activity within this range Leptospira interrogans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.3.21 2.41
-
 pyruvate
-
 Leptospira interrogans
2.3.3.21 2.41
-
 pyruvate recombinant enzyme, pH 8.0, 37°C Leptospira interrogans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.21 8
-
 assay at Leptospira interrogans

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.3.21 acetyl-CoA
-
 Leptospira interrogans