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Literature summary extracted from
- Evidence for the active role of a novel nuclease from Helicobacter pylori in the horizontal transfer of genetic information (2004), J. Bacteriol., 186, 2586-2593.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.21.1 | 2-mercaptoethanol | - |
Helicobacter pylori |  |
| 3.1.21.1 | dithiothreitol | - |
Helicobacter pylori | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.21.1 | expression in Escherichia coli as His-tag fusion construct | Helicobacter pylori |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.21.1 | 17000 | - |
SDS-PAGE | Helicobacter pylori |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.21.1 | Helicobacter pylori | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.21.1 | affinity chromatography | Helicobacter pylori |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.21.1 | cell culture | - |
Helicobacter pylori | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.21.1 | -20, 50 mM Tris-HCl 10 mM 2-mercatpoethanol 100 mM NaCl 10 mM EDTA 50% glycerol, several months, minimal loss of activity | Helicobacter pylori |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.21.1 | double-stranded circular DNA + H2O | - |
Helicobacter pylori | ? | - |
? | |
| 3.1.21.1 | double-stranded linear DNA + H2O | - |
Helicobacter pylori | ? | - |
? | |
| 3.1.21.1 | single-stranded DNA + H2O | - |
Helicobacter pylori | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.21.1 | dimer | 2 * 17000, gel filtration | Helicobacter pylori |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.21.1 | NucT | - |
Helicobacter pylori |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.21.1 | 8 | - |
- |
Helicobacter pylori |
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Release 2023.1 (January 2023)
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