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Literature summary extracted from

  • Kobayashi, T.; Shiraki, M.; Abe, T.; Sugiyama, A.; Saito, T.
    Purification and properties of an intracellular 3-hydroxybutyrate-oligomer hydrolase (PhaZ2) in Ralstonia eutropha H16 and its identification as a novel intracellular poly(3-hydroxybutyrate) depolymerase (2003), J. Bacteriol., 185, 3485-3490.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.75 His-tag, expression in Escherichia coli BLR(DE3)/pLysS Cupriavidus necator

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.75 intracellular associated with poly(3-hydroxybutyrate) granules Cupriavidus necator 5622
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O Cupriavidus necator specific for amorphous poly(3-hydroxybutyrate) poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.75 Cupriavidus necator
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.75 His-tag, Ni-affinity chromatography Cupriavidus necator

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.75 cell culture
-
 Cupriavidus necator
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O specific for amorphous poly(3-hydroxybutyrate) Cupriavidus necator poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O specific for amorphous poly(3-hydroxybutyrate) Cupriavidus necator poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x mainly 3-hydroxybutyrate oligomers ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.75 PhaZ1
-
 Cupriavidus necator
3.1.1.75 poly(3-hydroxybutyrate) depolymerase
-
 Cupriavidus necator