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Literature summary extracted from

  • Nakada, Y.; Itoh, Y.
    Characterization and regulation of the gbuA gene, encoding guanidinobutyrase in the arginine dehydrogenase pathway of Pseudomonas aeruginosa PAO1 (2002), J. Bacteriol., 184, 3377-3384.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.3.7 expression in Escherichia coli Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.7 49
-
 4-Guanidinobutyrate pH 9.5, 0.134 mM MnSO4, 37°C Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.7 34000
-
 4 * 34000, gel filtration, SDS-PAGE Pseudomonas aeruginosa
3.5.3.7 34700
-
 monomer, calculated from amino acid sequence Pseudomonas aeruginosa
3.5.3.7 140000
-
 gel filtration Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.7 4-Guanidinobutyrate + H2O Pseudomonas aeruginosa
-
 4-Aminobutyrate + urea
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.7 Pseudomonas aeruginosa
-
 PAO1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.7
-
 Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.7 4.7
-
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Pseudomonas aeruginosa 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O pH 9.5, 0.134 mM MnSO4, 37°C Pseudomonas aeruginosa 4-Aminobutyrate + urea
-
 ?
3.5.3.7 additional information no activity with arginine, agmatine, 3-guanidinopropionate and guanidinoacetate Pseudomonas aeruginosa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.7 tetramer 4 * 34000, gel filtration, SDS-PAGE Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
3.5.3.7 GBase
-
 Pseudomonas aeruginosa
3.5.3.7 guanidinobutyrase
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.3.7 1012
-
 4-Guanidinobutyrate pH 9.5, 0.134 mM MnSO4, 37°C Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.7 9.5
-
-
 Pseudomonas aeruginosa