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Literature summary extracted from
- Recombinant alpha-glucosidase from Aspergillus niger. Overexpression by Emericella nidulans, purification and characterization (2006), J. Appl. Glycosci., 53, 13-16.
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.20 | gene aglA, overexpression of the extracellular enzyme in Emericella nidulans strain JCM10259 | Aspergillus niger |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.20 | 0.59 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 4.5, 37°C, recombinant enzyme | Aspergillus niger |  |
| 3.2.1.20 | 0.62 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 4.5, 37°C, wild-type enzyme | Aspergillus niger | |
| 3.2.1.20 | 1.04 | - |
maltose | pH 4.5, 37°C, recombinant enzyme | Aspergillus niger | |
| 3.2.1.20 | 1.25 | - |
maltose | pH 4.5, 37°C, wild-type enzyme | Aspergillus niger | |
| 3.2.1.20 | 6.54 | - |
4-Nitrophenyl 2-deoxy-alpha-D-glucopyranoside | pH 4.5, 37°C, wild-type enzyme | Aspergillus niger | |
| 3.2.1.20 | 6.61 | - |
4-Nitrophenyl 2-deoxy-alpha-D-glucopyranoside | pH 4.5, 37°C, recombinant enzyme | Aspergillus niger | |
| 3.2.1.20 | 10.5 | - |
4-nitrophenyl 3-deoxy-alpha-D-glucopyranoside | pH 4.5, 37°C, wild-type enzyme | Aspergillus niger | |
| 3.2.1.20 | 11.6 | - |
4-nitrophenyl 3-deoxy-alpha-D-glucopyranoside | pH 4.5, 37°C, recombinant enzyme | Aspergillus niger | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 33000 | - |
1 * 98000 + 1 * 33000, recombinant enzyme, SDS-PAGE | Aspergillus niger |
| 3.2.1.20 | 98000 | - |
1 * 98000 + 1 * 33000, recombinant enzyme, SDS-PAGE | Aspergillus niger |
| 3.2.1.20 | 120000 | - |
recombinant enzyme, native PAGE | Aspergillus niger |
| 3.2.1.20 | 125000 | - |
wild-type enzyme, native PAGE | Aspergillus niger |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.20 | maltose + H2O | Aspergillus niger | - |
alpha-D-glucose + D-glucose | - |
? | |
| 3.2.1.20 | maltose + H2O | Aspergillus niger GN-3 | - |
alpha-D-glucose + D-glucose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.20 | Aspergillus niger | P56526 | gene aglA | - |
| 3.2.1.20 | Aspergillus niger GN-3 | P56526 | gene aglA | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.20 | glycoprotein | the wild-type enzyme contains over 35% more sugar than the recombinant enzyme | Aspergillus niger |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.20 | native enzyme and recombinant secreted extracellular enzyme from Emericella nidulans strain JCM10259 by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration to homogeneity | Aspergillus niger |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 0.13 | - |
purified recombinant enzyme, substrate 4-nitrophenyl 3-deoxy-alpha-D-glucopyranoside, recombinant enzyme | Aspergillus niger |
| 3.2.1.20 | 0.14 | - |
purified wild-type enzyme, substrate 4-nitrophenyl 3-deoxy-alpha-D-glucopyranoside, recombinant enzyme | Aspergillus niger |
| 3.2.1.20 | 0.96 | - |
purified recombinant enzyme, substrate 4-nitrophenyl alpha-D-glucopyranoside, recombinant enzyme | Aspergillus niger |
| 3.2.1.20 | 1.06 | - |
purified wild-type enzyme, substrate 4-nitrophenyl alpha-D-glucopyranoside, recombinant enzyme | Aspergillus niger |
| 3.2.1.20 | 3.7 | - |
purified recombinant enzyme, substrate 4-nitrophenyl 2-deoxy-alpha-D-glucopyranoside, recombinant enzyme | Aspergillus niger |
| 3.2.1.20 | 4.25 | - |
purified wild-type enzyme, substrate 4-nitrophenyl 2-deoxy-alpha-D-glucopyranoside, recombinant enzyme | Aspergillus niger |
| 3.2.1.20 | 46.8 | - |
purified recombinant enzyme, substrate maltose, recombinant enzyme | Aspergillus niger |
| 3.2.1.20 | 57.8 | - |
purified wild-type enzyme, substrate maltose, recombinant enzyme | Aspergillus niger |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.20 | 4-nitrophenyl 2-deoxy-alpha-D-glucopyranoside + H2O | low activity, wild-type and recombinant enzyme | Aspergillus niger | 4-nitrophenol + 2-deoxy-alpha-D-glucose | - |
? | |
| 3.2.1.20 | 4-nitrophenyl 2-deoxy-alpha-D-glucopyranoside + H2O | low activity, wild-type and recombinant enzyme | Aspergillus niger GN-3 | 4-nitrophenol + 2-deoxy-alpha-D-glucose | - |
? | |
| 3.2.1.20 | 4-nitrophenyl 3-deoxy-alpha-D-glucopyranoside + H2O | very low activity, wild-type and recombinant enzyme | Aspergillus niger | 4-nitrophenol + 3-deoxy-alpha-D-glucose | - |
? | |
| 3.2.1.20 | 4-nitrophenyl alpha-D-glucopyranoside + H2O | very low activity, wild-type and recombinant enzyme | Aspergillus niger | 4-nitrophenol + alpha-D-glucose | - |
? | |
| 3.2.1.20 | 4-nitrophenyl alpha-D-glucopyranoside + H2O | very low activity, wild-type and recombinant enzyme | Aspergillus niger GN-3 | 4-nitrophenol + alpha-D-glucose | - |
? | |
| 3.2.1.20 | maltose + H2O | - |
Aspergillus niger | alpha-D-glucose + D-glucose | - |
? | |
| 3.2.1.20 | maltose + H2O | preferred substrate of wild-type and recombinant enzyme | Aspergillus niger | alpha-D-glucose + D-glucose | - |
? | |
| 3.2.1.20 | maltose + H2O | - |
Aspergillus niger GN-3 | alpha-D-glucose + D-glucose | - |
? | |
| 3.2.1.20 | maltose + H2O | preferred substrate of wild-type and recombinant enzyme | Aspergillus niger GN-3 | alpha-D-glucose + D-glucose | - |
? | |
| 3.2.1.20 | additional information | no activity of wild-type and recombinant enzyme with 4-nitrophenyl 4-deoxy-alpha-D-glucopyranoside and 4-nitrophenyl 6-deoxy-alpha-D-glucopyranoside | Aspergillus niger | ? | - |
? | |
| 3.2.1.20 | additional information | no activity of wild-type and recombinant enzyme with 4-nitrophenyl 4-deoxy-alpha-D-glucopyranoside and 4-nitrophenyl 6-deoxy-alpha-D-glucopyranoside | Aspergillus niger GN-3 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.20 | dimer | 1 * 98000 + 1 * 33000, recombinant enzyme, SDS-PAGE | Aspergillus niger |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 37 | - |
wild-type and recombinant enzyme | Aspergillus niger |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 4.5 | - |
wild-type and recombinant enzyme | Aspergillus niger |
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