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Literature summary extracted from
- Expression of a cellobiohydrolase cDNA from the white rot fungus Irpex lacteus in Aspergillus oryzae (2005), J. Appl. Glycosci., 52, 23-26.
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.91 | gene cel2, expression of Ex-1 in Aspergillus oryzae, secretion of the recombinant enzyme to the culture fluid | Irpex lacteus |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.91 | 54733 | - |
x * 60000, recombinant Ex-1, SDS-PAGE, x * 54733, Ex-1, amino acid sequence calculation | Irpex lacteus |
| 3.2.1.91 | 60000 | - |
x * 60000, recombinant Ex-1, SDS-PAGE, x * 54733, Ex-1, amino acid sequence calculation | Irpex lacteus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.91 | Irpex lacteus | - |
white rot fungus, strain MC-2, gene cel2, exo-type cellobiohydrolase Ex-1 | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.91 | glycoprotein | Ex-1 contains two putative N-glycosylation sites, O-linked carbohydrates may also occur, glycosyl chains stabilize but are not essential for activity | Irpex lacteus |
| 3.2.1.91 | proteolytic modification | a putative N-terminal 18 amino acid signal peptide is removed | Irpex lacteus |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.91 | secreted recombinant Ex-1 from Aspergillus oryzae culture fluid by ammonium sulfate fractionation, cellulose adsorption chromatography, and gel filtration | Irpex lacteus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.91 | 20.9 | - |
native Ex-1, substrate Avicel | Irpex lacteus |
| 3.2.1.91 | 23.9 | - |
deglycosylated recombinant Ex-1, substrate Avicel | Irpex lacteus |
| 3.2.1.91 | 25 | - |
deglycosylated recombinant Ex-1, substrate carboxymethyl cellulose | Irpex lacteus |
| 3.2.1.91 | 26 | - |
native Ex-1, substrate carboxymethyl cellulose | Irpex lacteus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.91 | avicel + H2O | - |
Irpex lacteus | ? | - |
? |  |
| 3.2.1.91 | carboxymethyl cellulose + H2O | - |
Irpex lacteus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.91 | ? | x * 60000, recombinant Ex-1, SDS-PAGE, x * 54733, Ex-1, amino acid sequence calculation | Irpex lacteus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.91 | cellobiohydrolase | - |
Irpex lacteus |
| 3.2.1.91 | Ex-1 | - |
Irpex lacteus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.91 | 50 | - |
- |
Irpex lacteus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.91 | 5 | - |
- |
Irpex lacteus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.91 | 3 | 8 | recombinant Ex-1 | Irpex lacteus |
| 3.2.1.91 | 3 | 5 | native Ex-1 | Irpex lacteus |
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