Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kawatkar, S.P.; Kuntz, D.A.; Woods, R.J.; Rose, D.R.; Boons, G.J.
    Structural basis of the inhibition of Golgi alpha-mannosidase II by mannostatin A and the role of the thiomethyl moiety in ligand-protein interactions (2006), J. Am. Chem. Soc., 128, 8310-8319.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.114 enzyme in complex with inhibitors mannostatin A, N-benzyl mannostatin A, and 1-deoxyaminocyclopentitetrol, crystal growth overnight of enzyme solved in phosphate buffered reservoir solution, crystals are soaked for 3-6 h in 10 mM ligand solution, co-crystallization in Tris buffer without phosphate washing, X-ray diffraction structure determination and analysis, molecular modelling and structure simulation, overview Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.114 1-deoxyamino-cyclopentitetrol
-
Drosophila melanogaster
3.2.1.114 1-deoxyaminocyclopentitetrol structural basis of the inhibition of Golgi alpha-mannosidase II and the role of the thiomethyl moiety in ligand-protein interactions, overview Drosophila melanogaster
3.2.1.114 Mannostatin A structural basis of the inhibition of Golgi alpha-mannosidase II and the role of the thiomethyl moiety in ligand-protein interactions, overview Drosophila melanogaster
3.2.1.114 additional information simulations of ligand/inhibitor binding Drosophila melanogaster
3.2.1.114 N-benzyl mannostatin A structural basis of the inhibition of Golgi alpha-mannosidase II and the role of the thiomethyl moiety in ligand-protein interactions, overview Drosophila melanogaster
3.2.1.114 N-benzyl-mannostatin
-
Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.114 Golgi apparatus Golgi alpha-mannosidase II Drosophila melanogaster 5794
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.114 Drosophila melanogaster
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.114 method not mentioned Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.114 4-methylumbelliferyl alpha-D-mannoside + H2O
-
Drosophila melanogaster 4-methylumbelliferol + alpha-D-mannose
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.114 GmII
-
Drosophila melanogaster
3.2.1.114 Golgi alpha-mannosidase II
-
Drosophila melanogaster

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.114 0.000036
-
Mannostatin A
-
Drosophila melanogaster
3.2.1.114 0.000249
-
N-benzyl mannostatin A
-
Drosophila melanogaster
3.2.1.114 0.000249
-
N-benzyl-mannostatin
-
Drosophila melanogaster
3.2.1.114 0.265
-
1-deoxyaminocyclopentitetrol
-
Drosophila melanogaster
3.2.1.114 0.265
-
1-deoxyamino-cyclopentitetrol
-
Drosophila melanogaster