EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.2.1.114 | enzyme in complex with inhibitors mannostatin A, N-benzyl mannostatin A, and 1-deoxyaminocyclopentitetrol, crystal growth overnight of enzyme solved in phosphate buffered reservoir solution, crystals are soaked for 3-6 h in 10 mM ligand solution, co-crystallization in Tris buffer without phosphate washing, X-ray diffraction structure determination and analysis, molecular modelling and structure simulation, overview | Drosophila melanogaster |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.114 | 1-deoxyamino-cyclopentitetrol | - |
Drosophila melanogaster | |
3.2.1.114 | 1-deoxyaminocyclopentitetrol | structural basis of the inhibition of Golgi alpha-mannosidase II and the role of the thiomethyl moiety in ligand-protein interactions, overview | Drosophila melanogaster | |
3.2.1.114 | Mannostatin A | structural basis of the inhibition of Golgi alpha-mannosidase II and the role of the thiomethyl moiety in ligand-protein interactions, overview | Drosophila melanogaster | |
3.2.1.114 | additional information | simulations of ligand/inhibitor binding | Drosophila melanogaster | |
3.2.1.114 | N-benzyl mannostatin A | structural basis of the inhibition of Golgi alpha-mannosidase II and the role of the thiomethyl moiety in ligand-protein interactions, overview | Drosophila melanogaster | |
3.2.1.114 | N-benzyl-mannostatin | - |
Drosophila melanogaster |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.114 | Golgi apparatus | Golgi alpha-mannosidase II | Drosophila melanogaster | 5794 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.114 | Drosophila melanogaster | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.114 | method not mentioned | Drosophila melanogaster |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.114 | 4-methylumbelliferyl alpha-D-mannoside + H2O | - |
Drosophila melanogaster | 4-methylumbelliferol + alpha-D-mannose | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.114 | GmII | - |
Drosophila melanogaster |
3.2.1.114 | Golgi alpha-mannosidase II | - |
Drosophila melanogaster |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.114 | 0.000036 | - |
Mannostatin A | - |
Drosophila melanogaster | |
3.2.1.114 | 0.000249 | - |
N-benzyl mannostatin A | - |
Drosophila melanogaster | |
3.2.1.114 | 0.000249 | - |
N-benzyl-mannostatin | - |
Drosophila melanogaster | |
3.2.1.114 | 0.265 | - |
1-deoxyaminocyclopentitetrol | - |
Drosophila melanogaster | |
3.2.1.114 | 0.265 | - |
1-deoxyamino-cyclopentitetrol | - |
Drosophila melanogaster |