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Literature summary extracted from

  • Auh, J.H.; Chae, H.Y.; Kim, Y.R.; Shim, K.H.; Yoo, S.H.; Park, K.H.
    Modification of rice starch by selective degradation of amylose using alkalophilic Bacillus cyclomaltodextrinase (2006), J. Agric. Food Chem., 54, 2314-2319.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.54 synthesis CDase I-5 is applied to modify the starch structure to produce low-amylose starch products by incubating rice starch with this enzyme. The amylose content of rice starch decreases from 28.5 to 9% while the amylopectin content remains almost constant with no significant change in side chain length distribution Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.54 additional information
-
 additional information
-
 Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.54 64000
-
 12 * 64000, assembly of six dimers, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.54 Bacillus sp. (in: Bacteria)
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.54
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.54 amylopectin + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
3.2.1.54 amylose + H2O CDase I-5 shows high preference towards amylose Bacillus sp. (in: Bacteria) maltose + glucose major hydrolysis products ?
3.2.1.54 cyclomaltoheptaose + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.54 dodecamer 12 * 64000, assembly of six dimers, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

EC Number Synonyms Comment Organism
3.2.1.54 CDase I-5
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.54 50
-
 substrate: cyclomaltoheptaose Bacillus sp. (in: Bacteria)

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.54 30 60 30°C: about 45% of maximal activity, 60°C: about 80% of maximal activity, substrate: cyclomaltoheptaose Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.54 0.19
-
 amylopectin
-
 Bacillus sp. (in: Bacteria)
3.2.1.54 2 8 amylose
-
 Bacillus sp. (in: Bacteria)
3.2.1.54 3
-
 cyclomaltoheptaose
-
 Bacillus sp. (in: Bacteria)
3.2.1.54 22.11
-
 amylose
-
 Bacillus sp. (in: Bacteria)
3.2.1.54 50.36
-
 amylopectin
-
 Bacillus sp. (in: Bacteria)
3.2.1.54 78.18
-
 cyclomaltoheptaose
-
 Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.54 7.5
-
 substrate: cyclomaltoheptaose Bacillus sp. (in: Bacteria)

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.54 6.5 8.5 pH 6.5: about 50% of maximal activity, pH 8.5: about 35% of maximal activity, substrate: cyclomaltoheptaose Bacillus sp. (in: Bacteria)