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Literature summary extracted from
- Vacuolar invertases in sweet potato: molecular cloning, characterization, and analysis of gene expression (2005), J. Agric. Food Chem., 53, 3672-3678.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.26 | gene lbbetafruct2, DNA and amino acid sequence determination and analysis, expression of the His-tagged soluble, vacuolar isozyme in Pichia pastoris | Ipomoea batatas |
| 3.2.1.26 | gene lbbetafruct3, DNA and amino acid sequence determination and analysis, expression of the His-tagged soluble, vacuolar isozyme in Pichia pastoris | Ipomoea batatas |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.26 | 4.97 | - |
sucrose | pH 5.0, recombinant isozyme lbbetafruct3 | Ipomoea batatas |  |
| 3.2.1.26 | 10.1 | - |
sucrose | pH 5.0, recombinant isozyme lbbetafruct2 | Ipomoea batatas | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.26 | soluble | vacuolar acid invertase | Ipomoea batatas | - |
- |
| 3.2.1.26 | vacuole | acid invertase | Ipomoea batatas | 5773 | - |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.26 | Ipomoea batatas | Q94C05 | isozyme lbbetafruct3; cv. Tainong 57 | - |
| 3.2.1.26 | Ipomoea batatas | Q94C06 | isozyme lbbetafruct2; cv. Tainong 57 | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.26 | glycoprotein | deglycosylation by PMGase F, the glycosylation of isozyme lbbetafruct2 differs in the different plant tissues and between native and recombinant enzyme, overview | Ipomoea batatas |
| 3.2.1.26 | glycoprotein | deglycosylation by PMGase F, the glycosylation of isozyme lbbetafruct3 differs in the different plant tissues and between native and recombinant enzyme, overview | Ipomoea batatas |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.26 | recombinant His-tagged soluble, vacuolar isozyme lbbetafruct3 5fold from Pichia pastoris by nickel affinity chromatography | Ipomoea batatas |
| 3.2.1.26 | recombinant His-tagged soluble, vacuolar isozyme lbbetalbbetafruct2 7.6fold from Pichia pastoris by nickel affinity chromatography | Ipomoea batatas |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.26 | leaf | young and mature, expression of isozyme lbbetafruct3 is restricted to shoots and leaves | Ipomoea batatas | - |
| 3.2.1.26 | additional information | isozyme lbbetafruct2 is expressed in most tissues, except for mature leaves and sprouting storage roots, exspecially in sink organs | Ipomoea batatas | - |
| 3.2.1.26 | shoot | expression of isozyme lbbetafruct3 is restricted to shoots and leaves | Ipomoea batatas | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.26 | 70 | - |
purified recombinant isozyme lbbetafruct3 | Ipomoea batatas |
| 3.2.1.26 | 553 | - |
purified recombinant isozyme lbbetafruct2 | Ipomoea batatas |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.26 | additional information | activity with cellobiose, maltose, lactose, and inulin, by isozyme lbbetafruct2 | Ipomoea batatas | ? | - |
? | |
| 3.2.1.26 | additional information | activity with cellobiose, maltose, lactose, and inulin, by isozyme lbbetafruct3 | Ipomoea batatas | ? | - |
? | |
| 3.2.1.26 | raffinose + H2O | 25% of the activity with sucrose, recombinant isozyme lbbetafruct3 | Ipomoea batatas | alpha-D-galactosyl-1,6-alpha-D-glucose + D-fructose | - |
? | |
| 3.2.1.26 | raffinose + H2O | 37% of the activity with sucrose, recombinant isozyme lbbetafruct2 | Ipomoea batatas | alpha-D-galactosyl-1,6-alpha-D-glucose + D-fructose | - |
? | |
| 3.2.1.26 | stachyose + H2O | 13% of the activity with sucrose, recombinant isozyme lbbetafruct2 | Ipomoea batatas | ? | - |
? | |
| 3.2.1.26 | stachyose + H2O | 9% of the activity with sucrose, recombinant isozyme lbbetafruct3 | Ipomoea batatas | ? | - |
? | |
| 3.2.1.26 | sucrose + H2O | preferred substrate, recombinant isozyme lbbetafruct2 | Ipomoea batatas | D-glucose + D-fructose | - |
? | |
| 3.2.1.26 | sucrose + H2O | preferred substrate, recombinant isozyme lbbetafruct3 | Ipomoea batatas | D-glucose + D-fructose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.26 | More | analysis of conserved notifs and segments in the secondray structure of isozyme lbbetafruct2, overview | Ipomoea batatas |
| 3.2.1.26 | More | analysis of conserved notifs and segments in the secondray structure of isozyme lbbetafruct3, overview | Ipomoea batatas |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.26 | lbbetafruct2 | - |
Ipomoea batatas |
| 3.2.1.26 | lbbetafruct3 | - |
Ipomoea batatas |
| 3.2.1.26 | soluble acid invertase | - |
Ipomoea batatas |
| 3.2.1.26 | Vacuolar invertase | - |
Ipomoea batatas |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.26 | 5 | - |
recombinant isozyme lbbetafruct2 | Ipomoea batatas |
| 3.2.1.26 | 5 | - |
recombinant isozyme lbbetafruct3 | Ipomoea batatas |
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