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Literature summary extracted from
- Characterization of two Acanthoscelides obtectus alpha-amylases and their inactivation by wheat inhibitors (2005), J. Agric. Food Chem., 53, 1585-1590.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.1 | alpha-AI1 | alpha-amylase inhibitor 1 from cultivated Phaseolus vulgaris | Acanthoscelides obtectus | |
| 3.2.1.1 | alpha-AI2 | alpha-amylase inhibitor 2 from wild Phaseolus vulgaris | Acanthoscelides obtectus | |
| 3.2.1.1 | wheat alpha-amylase inhibitor 0.19 | inhibitor 0.19 isolated from wheat kernels, inhibits both isozymes AoA1 and AoA2 in vitro and in vivo, stoichiometry of inhibition of isozymes, biological activity of inhibitor 0.19, overview | Acanthoscelides obtectus | |
| 3.2.1.1 | wheat alpha-amylase inhibitor 0.35 | inhibitor 0.35 isolated from wheat kernels, inhibits both isozymes AoA1 and AoA2 in vitro and in vivo, stoichiometry of inhibition of isozymes, biological activity of inhibitor 0.53, overview | Acanthoscelides obtectus |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.1 | Ca2+ | - |
Acanthoscelides obtectus |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 30000 | - |
isozyme AoA1, gel filtration | Acanthoscelides obtectus |
| 3.2.1.1 | 45000 | - |
isozyme AoA2, gel filtration | Acanthoscelides obtectus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.1 | Acanthoscelides obtectus | - |
two isozymes AoA1 and AoA2 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.1 | native isozymes AoA1 and AoA2 from larval gut 30.5fold and 65.4fold, respectively, by cation exchange and beta-cyclodextrin affinity chromatography, and gel filtration | Acanthoscelides obtectus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.1 | gut | - |
Acanthoscelides obtectus | - |
| 3.2.1.1 | larva | - |
Acanthoscelides obtectus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | additional information | - |
purified isozyme AoA1 and isozyme AoA2 | Acanthoscelides obtectus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.1 | starch + H2O | soluble starch | Acanthoscelides obtectus | malto-oligosaccharides | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.1 | monomer | 1 * 30000, isozyme AoA1, SDS-PAGE, 1 * 45000, isozyme AoA2, SDS-PAGE | Acanthoscelides obtectus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.1 | AoA1 | - |
Acanthoscelides obtectus |
| 3.2.1.1 | AoA2 | - |
Acanthoscelides obtectus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 5.8 | - |
assay at | Acanthoscelides obtectus |
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Release 2023.1 (January 2023)
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