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Literature summary extracted from

  • Suzuki, T.; Honda, Y.; Mukasa, Y.
    Purification and characterization of lipase in buckwheat seed (2004), J. Agric. Food Chem., 52, 7407-7411.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 26500
-
 isozyme LIP II, gel filtration Fagopyrum esculentum
3.1.1.3 28400
-
 1 * 28400, isozyme LIP II, SDS-PAGE, 1 * 150000, isozyme LIP I, SDS-PAGE Fagopyrum esculentum
3.1.1.3 150000
-
 1 * 28400, isozyme LIP II, SDS-PAGE, 1 * 150000, isozyme LIP I, SDS-PAGE Fagopyrum esculentum
3.1.1.3 171000
-
 isozyme LIP I, gel filtration Fagopyrum esculentum

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Fagopyrum esculentum
-
 var. Kitawasesoba, two isozymes LIP I and LIP II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 native isozymes LIP I and LIP II 60fold and 143fold from seed to near homogeneity by ion exchange chromatography and gel filtration, respectively Fagopyrum esculentum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.3 embryo high activity Fagopyrum esculentum
-
3.1.1.3 endosperm low activity compare to the embryo Fagopyrum esculentum
-
3.1.1.3 testis very low activity Fagopyrum esculentum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 0.11
-
 purified isozyme LIP I, substrate triolein, 30°C Fagopyrum esculentum
3.1.1.3 0.36
-
 purified isozyme LIP I, substrate 4-nitrophenyl laurate, 22°C Fagopyrum esculentum
3.1.1.3 0.73
-
 purified isozyme LIP II, substrate triolein, 30°C Fagopyrum esculentum
3.1.1.3 0.86
-
 purified isozyme LIP II, substrate 4-nitrophenyl laurate, 22°C Fagopyrum esculentum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl acetate + H2O
-
 Fagopyrum esculentum 4-nitrophenol + acetate
-
 ?
3.1.1.3 4-nitrophenyl butyrate + H2O
-
 Fagopyrum esculentum 4-nitrophenol + butyrate
-
 ?
3.1.1.3 4-nitrophenyl caproate + H2O
-
 Fagopyrum esculentum 4-nitrophenol + caproate
-
 ?
3.1.1.3 4-nitrophenyl laurate + H2O best substrate Fagopyrum esculentum 4-nitrophenol + laurate
-
 ?
3.1.1.3 olein + H2O low activity with isozyme LIP II, residual activity with isozyme LIP I Fagopyrum esculentum glycerol + oleate
-
 ?
3.1.1.3 palmitin + H2O low activity with isozyme LIP II, residual activity with isozyme LIP I Fagopyrum esculentum glycerol + palmitate
-
 ?
3.1.1.3 triolein + H2O good substrate Fagopyrum esculentum diolein + oleate
-
 ?
3.1.1.3 tripalmitin + H2O low activity with isozyme LIP II, residual activity with isozyme LIP I Fagopyrum esculentum dipalmitin + palmitate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 monomer 1 * 28400, isozyme LIP II, SDS-PAGE, 1 * 150000, isozyme LIP I, SDS-PAGE Fagopyrum esculentum

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 LIP
-
 Fagopyrum esculentum
3.1.1.3 lipase
-
 Fagopyrum esculentum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 30
-
 isozyme LIP I, and isozyme LIP I with substrate triolein Fagopyrum esculentum
3.1.1.3 40
-
 isozyme LIP I with substrate 4-nitrophenyl laurate Fagopyrum esculentum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 10 70 isozymes LIP I amd II, activity is dependent on substrate, fully stable at 10°C and 45% remaining activity at 70°C with substrate triolein after 4 h Fagopyrum esculentum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 3
-
 isozyme LIP I with substrate triolein Fagopyrum esculentum
3.1.1.3 5
-
 isozyme LIP I with substrate 4-nitrophenyl laurate Fagopyrum esculentum
3.1.1.3 6
-
 isozyme LIP II Fagopyrum esculentum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.3 3 8 isozymes LIP I and II with substrate 4-nitrophenyl laurate Fagopyrum esculentum
3.1.1.3 3 7 isozymes LIP I and II with substrate triolein Fagopyrum esculentum