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Literature summary extracted from

  • Noguchi, Y.; Nagata, H.; Koganei, H.; Kodera, Y.; Hiroto, M.; Nishimura, H.; Inada, Y.; Matsushima, A.
    Isolation and characterization of aminopeptidase (Jc-peptidase) from Japanese cedar pollen (Cryptomeria japonica) (2002), J. Agric. Food Chem., 50, 3540-3543.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.22 amastatin
-
 Cryptomeria japonica
3.4.11.22 bestatin i.e. [(2S,3R)-3-amino-2-hydroxy-4-phenylbutanoyl-L-valyl]-L-leucine Cryptomeria japonica
3.4.11.22 additional information leuhistin, arphamenine A and B, and actinonin are poor inhibitors Cryptomeria japonica
3.4.11.22 phebestin i.e. [(2S,3R)-3-amino-2-hydroxy-4-phenylbutanoyl-L-valyl]-L-phenylalanine Cryptomeria japonica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.22 42000
-
 1 * 42000, SDS-PAGE Cryptomeria japonica
3.4.11.22 43000
-
 gel filtration Cryptomeria japonica

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.22 Cryptomeria japonica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.22 native enzyme 96fold from pollen by a seven-step procedure comprising ammonium sulfate fractionation, 2 steps of anion exchange chromatography, gel filtration, and hydrophobic interaction chromatography Cryptomeria japonica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.22 pollen
-
 Cryptomeria japonica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.22 6.7
-
 purified enzyme Cryptomeria japonica

Storage Stability

EC Number Storage Stability Organism
3.4.11.22 4°C, aqueous enzyme solution, in presence of Brij 35, pH 6.0-9.0, stable for a few days Cryptomeria japonica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.22 L-Leu-7-amido-4-methylcoumarin + H2O
-
 Cryptomeria japonica L-Leu + 7-amino-4-methylcoumarin
-
 ?
3.4.11.22 L-Met-7-amido-4-methylcoumarin + H2O no activity with Z-Met-7-amido-4-methylcoumarin Cryptomeria japonica L-Met + 7-amino-4-methylcoumarin
-
 ?
3.4.11.22 L-Phe-7-amido-4-methylcoumarin + H2O
-
 Cryptomeria japonica L-Phe + 7-amino-4-methylcoumarin
-
 ?
3.4.11.22 L-Tyr-7-amido-4-methylcoumarin + H2O
-
 Cryptomeria japonica L-Tyr + 7-amino-4-methylcoumarin
-
 ?
3.4.11.22 additional information substrate specificity, overview, no activity with L-Arg-7-amido-4-methylcoumarin and L-Glu-7-amido-4-methylcoumarin Cryptomeria japonica ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.22 monomer 1 * 42000, SDS-PAGE Cryptomeria japonica

Synonyms

EC Number Synonyms Comment Organism
3.4.11.22 Jc-peptidase
-
 Cryptomeria japonica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.22 37
-
 assay at Cryptomeria japonica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.22 7.3
-
-
 Cryptomeria japonica

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.11.22 0.047
-
 phebestin pH 7.3, 37°C Cryptomeria japonica
3.4.11.22 0.11
-
 bestatin pH 7.3, 37°C Cryptomeria japonica