Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • McCarthy, E.; Stack, C.; Donnelly, S.M.; Doyle, S.; Mann, V.H.; Brindley, P.J.; Stewart, M.; Day, T.A.; Maule, A.G.; Dalton, J.P.
    Leucine aminopeptidase of the human blood flukes, Schistosoma mansoni and Schistosoma japonicum (2004), Int. J. Parasitol., 34, 703-714.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.1 DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional expression of His6-tagged enzyme in Spodoptera frugiperda Sf9 insect cells using the baculovirus transfection system Schistosoma mansoni

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.1 1,10-phenanthroline 61.5% inhibition at 5 mM Schistosoma japonicum
3.4.11.1 1,10-phenanthroline 71% inhibition at 5 mM Schistosoma mansoni
3.4.11.1 bestatin nearly complete inhibition at 0.05 mM Schistosoma japonicum
3.4.11.1 bestatin nearly complete inhibition at 0.05 mM Schistosoma mansoni
3.4.11.1 EDTA 50% inhibition at 5 mM Schistosoma mansoni
3.4.11.1 additional information no inhibition by EDTA Schistosoma japonicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.1 soluble
-
 Schistosoma mansoni
-
-
3.4.11.1 soluble
-
 Schistosoma japonicum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.1 Mg2+ about 2fold activation at 0.5 mM for the recombinant enzyme and about 4fold for the wild-type enzyme in cell extract, metallopeptidase Schistosoma mansoni
3.4.11.1 Mg2+ about 3.5fold activation at 0.5 mM, metallopeptidase Schistosoma japonicum
3.4.11.1 Mn2+ about 2fold activation at 0.05 mM for the recombinant enzyme and about 4fold for the wild-type enzyme in cell extract, metallopeptidase Schistosoma mansoni
3.4.11.1 Mn2+ about 4fold activation at 0.05 mM, metallopeptidase Schistosoma japonicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.1 57500
-
 x * 57500, recombinant enzyme, SDS-PAGE Schistosoma mansoni

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Schistosoma japonicum Q9GQ37 incomplete sequence missing 26 N-terminal amino acids
-
3.4.11.1 Schistosoma mansoni
-
 synthetic construct
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.1 recombinant His6-tagged enzyme from Spodoptera frugiperda Sf9 insect cells by nickel affinity chromatography Schistosoma mansoni

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.11.1 release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid mides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low endopeptidase Schistosoma mansoni
a
3.4.11.1 release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid mides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low endopeptidase Schistosoma japonicum
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.1 gut gastrodermal cells surrounding the gut lumen Schistosoma mansoni
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.1 additional information
-
-
 Schistosoma mansoni
3.4.11.1 additional information
-
-
 Schistosoma japonicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.1 L-alanyl-7-amido-4-methylcoumarin + H2O
-
 Schistosoma mansoni L-alanine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 L-alanyl-7-amido-4-methylcoumarin + H2O
-
 Schistosoma japonicum L-alanine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 L-leucyl-7-amido-4-methylcoumarin + H2O best substrate Schistosoma mansoni L-leucine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 L-leucyl-7-amido-4-methylcoumarin + H2O best substrate Schistosoma japonicum L-leucine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 L-prolyl-7-amido-4-methylcoumarin + H2O
-
 Schistosoma mansoni L-proline + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 L-prolyl-7-amido-4-methylcoumarin + H2O
-
 Schistosoma japonicum L-proline + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 L-tyrosyl-7-amido-4-methylcoumarin + H2O
-
 Schistosoma mansoni L-tyrosine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 L-tyrosyl-7-amido-4-methylcoumarin + H2O
-
 Schistosoma japonicum L-tyrosine + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.1 ? x * 57500, recombinant enzyme, SDS-PAGE Schistosoma mansoni

Synonyms

EC Number Synonyms Comment Organism
3.4.11.1 LAP
-
 Schistosoma mansoni
3.4.11.1 LAP
-
 Schistosoma japonicum
3.4.11.1 leucine aminopeptidase
-
 Schistosoma mansoni
3.4.11.1 leucine aminopeptidase
-
 Schistosoma japonicum
3.4.11.1 More the enzyme belongs to the M17 leucine peptidase family Schistosoma mansoni
3.4.11.1 More the enzyme belongs to the M17 leucine peptidase family Schistosoma japonicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.1 37
-
 assay at Schistosoma mansoni
3.4.11.1 37
-
 assay at Schistosoma japonicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.1 8.25
-
-
 Schistosoma mansoni
3.4.11.1 8.25
-
-
 Schistosoma japonicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.1 6.5 9.4 no activity below pH 6.5 Schistosoma mansoni