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Literature summary extracted from

  • Ichikawa, E.; Tsurusaki, Y.; Yamaguchi, M.
    Suppressive effect of regucalcin on protein phosphatase activity in the heart cytosol of normal and regucalcin transgenic rats (2004), Int. J. Mol. Med., 13, 289-293.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.16 additional information construction of regucalcin-expressing transgenic Sprague-Dawley rats showing reduced protein phosphatase activity in the heart cytosol, overview Rattus norvegicus
3.1.3.16 additional information construction of transgenic rats expressing the inhibitory protein regucalcin in the heart muscle cell cytosol leading to reduced enzyme activity and abolishing the activating effect of Ca2+, overview Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.16 regucalcin has regulatory function in heart cytosol; the protein inhibits the enzyme in presence of EGTA or ethylene glycol and has a regulatory function for the enzyme independently of Ca2+ Rattus norvegicus
3.1.3.16 trifluoperazin cocmplete inhibition at 0.001-0.002 mM Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.16 cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.16 Ca2+ activates Rattus norvegicus
3.1.3.16 Ca2+ activates, synergistic with calmodulin, activation is completely inhibited by trifluoroperazine Rattus norvegicus
3.1.3.16 Calmodulin activates the enzyme with substratees phospho-L-serine and phospho-L-threonine, but not with phospho-L-tyrosine Rattus norvegicus
3.1.3.16 Mg2+ activates Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.16 phospho-L-serine + H2O Rattus norvegicus
-
 L-serine + phosphate
-
 ?
3.1.3.16 phospho-L-threonine + H2O Rattus norvegicus
-
 L-threonine + phosphate
-
 ?
3.1.3.16 phospho-L-tyrosine + H2O Rattus norvegicus preferred substrate L-tyrosine + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.16 Rattus norvegicus
-
 female and male Wistar rats
-
3.1.3.16 Rattus norvegicus
-
 regucalcin-expressing transgenic Sprague-Dawley rats and wild-type male Wistar rats
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.16 cardiac muscle
-
 Rattus norvegicus
-
3.1.3.16 heart
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.16 additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.16 phospho-L-serine + H2O
-
 Rattus norvegicus L-serine + phosphate
-
 ?
3.1.3.16 phospho-L-threonine + H2O
-
 Rattus norvegicus L-threonine + phosphate
-
 ?
3.1.3.16 phospho-L-tyrosine + H2O
-
 Rattus norvegicus L-tyrosine + phosphate
-
 ?
3.1.3.16 phospho-L-tyrosine + H2O preferred substrate Rattus norvegicus L-tyrosine + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.16 More cf. EC 3.1.3.48, protein-tyrosine phosphatase Rattus norvegicus
3.1.3.16 More cf. EC 3.1.3.48, protein-tyrosine-phosphatase Rattus norvegicus
3.1.3.16 protein phosphatase
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.16 30
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.16 8
-
 assay at Rattus norvegicus