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Literature summary extracted from

  • Niaz, M.; Ghafoor, M.Y.; Jabbar, A.; Wahid, A.; Rasul, E.; Ahmed, R.; Rashid, M.H.
    Properties of glucoamylase from a mesophilic fungus Arachniotus citrinus produced under solid-state growth condition (2004), Int. J. Biol. Biotechnol., 1, 223-231.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.3 industry the enzyme can be useful in industrial processing and hydrolysis of starch Gymnascella citrina

General Stability

EC Number General Stability Organism
3.2.1.3 the purified enzyme is sensitive to proteolytic degradation by alpha-chymotrypsin, half-life 39 min Gymnascella citrina

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.3 additional information the purified enzyme is sensitive to proteolytic degradation by alpha-chymotrypsin Gymnascella citrina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.3 0.05
-
 starch pH 3.5-5.5, 50°C Gymnascella citrina

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.3 starch + H2O Gymnascella citrina
-
 starch + beta-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Gymnascella citrina
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.3 native enzyme 63fold to homogeneity by ammonium sulfate precipitation, anion exchange and hydrophobic interaction chromatography, and again anion exchange chromatography Gymnascella citrina

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.3 additional information solid-state culture on wheat bran Gymnascella citrina
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 starch + H2O
-
 Gymnascella citrina starch + beta-D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.3 exo-1,4-alpha-D-glucan glucanohydrolase
-
 Gymnascella citrina
3.2.1.3 glucoamylase
-
 Gymnascella citrina

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 35
-
 at 0.39 mg/ml purified enzyme Gymnascella citrina
3.2.1.3 55
-
 at 1 mg/ml purified enzyme Gymnascella citrina

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.3 55
-
 half-life of purified enzyme is 7 min, irreversible inactivation Gymnascella citrina

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.3 8.3
-
 starch pH 3.5-5.5, 50°C Gymnascella citrina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 3.5 5.5
-
 Gymnascella citrina