Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hale, L.P.; Greer, P.K.; Trinh, C.T.; James, C.L.
    Proteinase activity and stability of natural bromelain preparations (2005), Int. Immunopharmacol., 5, 783-793.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.22.33 incubation with trypsin (1 mg/ml, 1 h, 37°C) results in 12% loss of activity (with benzyloxycarbonyl-Arg-Arg-p-nitroanilide as substrate) at bromelain concentration of 10 mg/ml , but has no effect on activity at bromelain concentrations of 100 mg/ml Ananas comosus
3.4.22.33 proteolytic activity of solutions containing either 10 mg/ml or 100 mg/ml bromelain in water or 100 mg/ml NaHCO3 is not changed against benzyloxycarbonyl-Arg-Arg-p-nitroanilide and benzoyl-Phe-Val-Arg-p-nitroanilide substrate by four freeze/thaw cycles Ananas comosus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.33 Ananas comosus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.33 fruit
-
 Ananas comosus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.33 benzoyl-Phe-Val-Arg-p-nitroanilide + H2O
-
 Ananas comosus benzoyl-Phe-Val-Arg + p-nitroaniline
-
 ?
3.4.22.33 benzyloxycarbonyl-Arg-Arg-p-nitroanilide + H2O
-
 Ananas comosus benzyloxycarbonyl-Arg-Arg + p-nitroaniline
-
 ?

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.33 2
-
 at a concentration of 250 mg/ml the enzyme is resistant to inactivation Ananas comosus