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Literature summary extracted from

  • Fernandez, J.; Mohedano, A.F.; Gaya, P.; Medina, M.; Nunez, M.
    Purification and properties of two intracellular aminopeptidases produced by Brevibacterium linens SR3 (2000), Int. Dairy J., 10, 241-248.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.10 1,10-phenanthroline strong inhibition Brevibacterium linens
3.4.11.10 Ca2+
-
 Brevibacterium linens
3.4.11.10 Cu2+
-
 Brevibacterium linens
3.4.11.10 cysteine strong inhibition Brevibacterium linens
3.4.11.10 EDTA strong inhibition Brevibacterium linens
3.4.11.10 Fe2+
-
 Brevibacterium linens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.10 0.25
-
 L-Leu-4-nitroanilide pH 8.0, 37°C, APII Brevibacterium linens
3.4.11.10 1
-
 L-Leu-4-nitroanilide pH 8.5, 45°C, API Brevibacterium linens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.10 intracellular
-
 Brevibacterium linens 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.10 Co2+ activates Brevibacterium linens
3.4.11.10 additional information API and APII are metallopeptidases Brevibacterium linens
3.4.11.10 Ni2+ activates Brevibacterium linens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.10 14000
-
 x * 14000, API, SDS-PAGE, x * 18000, APII, SDS-PAGE Brevibacterium linens
3.4.11.10 18000
-
 x * 14000, API, SDS-PAGE, x * 18000, APII, SDS-PAGE Brevibacterium linens
3.4.11.10 80000
-
 API, gel filtration Brevibacterium linens
3.4.11.10 220000
-
 APII, gel filtration Brevibacterium linens

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.10 Brevibacterium linens
-
-
-
3.4.11.10 Brevibacterium linens SR3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.10 native API and APII to homogeneity by hydrophobic interaction and ion exchange chromatography, and gel filtration Brevibacterium linens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.10 L-Leu-4-nitroanilide + H2O
-
 Brevibacterium linens L-Leu + 4-nitroaniline
-
 ?
3.4.11.10 L-Leu-4-nitroanilide + H2O
-
 Brevibacterium linens SR3 L-Leu + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.10 oligomer x * 14000, API, SDS-PAGE, x * 18000, APII, SDS-PAGE Brevibacterium linens

Synonyms

EC Number Synonyms Comment Organism
3.4.11.10 API
-
 Brevibacterium linens
3.4.11.10 APII
-
 Brevibacterium linens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.10 37
-
 APII Brevibacterium linens
3.4.11.10 45
-
 API Brevibacterium linens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.10 8
-
 APII Brevibacterium linens
3.4.11.10 8.5
-
 API Brevibacterium linens