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Literature summary extracted from

  • Daimon, T.; Katsuma, S.; Iwanaga, M.; Kang, W.; Shimada, T.
    The BmChi-h gene, a bacterial-type chitinase gene of Bombyx mori, encodes a functional exochitinase that plays a role in the chitin degradation during the molting process (2005), Insect Biochem. Mol. Biol., 35, 1112-1123.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 DNA and amino acid sequence determination and analysis, phylogenetic analysis Antheraea pernyi
3.2.1.14 DNA and amino acid sequence determination and analysis, phylogenetic analysis Ostrinia furnacalis
3.2.1.14 DNA and amino acid sequence determination and analysis, phylogenetic analysis Agrius convolvuli
3.2.1.14 DNA and amino acid sequence determination and analysis, phylogenetic analysis Mythimna separata
3.2.1.14 DNA and amino acid sequence determination and analysis, phylogenetic analysis Samia cynthia
3.2.1.14 gene Chi-h, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged wild-type and mutant Chi-h in Spodoptera frugiperda Sf9 cells using the Autographa californica nucleopolyhedrovirus for transfection constructed in a Bac-to-Bac system Bombyx mori

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.14 D306N site-directed mutagenesis using different sets of primers, the mutant enzyme shows reduced activity and high pH sensitivity compared to the wild-type enzyme Bombyx mori
3.2.1.14 D306N/D310Q site-directed mutagenesis using different sets of primers, inactive mutant Bombyx mori
3.2.1.14 D310Q site-directed mutagenesis using different sets of primers, inactive mutant Bombyx mori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.14 additional information Bombyx mori Chi-h plays a role in the chitin degradation during the molting process ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Agrius convolvuli Q4AE58
-
-
3.2.1.14 Antheraea pernyi Q4AE61
-
-
3.2.1.14 Bombyx mori Q869E2 gene Chi-h encodes an exochitinase, the organism also contains an endochitinase
-
3.2.1.14 Mythimna separata Q4AE57 i.e. Mythimna separata
-
3.2.1.14 Ostrinia furnacalis Q4AE59
-
-
3.2.1.14 Samia cynthia Q4AE60
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 recombinant His-tagged wild-type and mutant Chi-h from Sf9 insect cells by metal affinity chromatography and dialysis Bombyx mori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside + H2O
-
Bombyx mori 4-methylumbelliferol + beta-N,N',N''-triacetylchitotriose
-
?
3.2.1.14 4-methylumbelliferyl beta-N,N'-diacetylchitobioside + H2O
-
Bombyx mori 4-methylumbelliferol + beta-N,N'-diacetylchitobiose
-
?
3.2.1.14 4-methylumbelliferyl beta-N-acetyl-D-glucosamine + H2O
-
Bombyx mori 4-methylumbelliferol + beta-N-acetyl-D-glucosamine
-
?
3.2.1.14 chitin oligosaccharides + H2O glycol chitin assay method, (GlcNAc)1-6 Bombyx mori N-acetyl glucosamine
-
?
3.2.1.14 additional information Chi-h plays a role in the chitin degradation during the molting process Bombyx mori ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 bacterial-type chitinase
-
Bombyx mori
3.2.1.14 Chi-h
-
Antheraea pernyi
3.2.1.14 Chi-h
-
Ostrinia furnacalis
3.2.1.14 Chi-h
-
Agrius convolvuli
3.2.1.14 Chi-h
-
Mythimna separata
3.2.1.14 Chi-h
-
Bombyx mori
3.2.1.14 Chi-h
-
Samia cynthia
3.2.1.14 exochitinase
-
Bombyx mori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 25
-
assay at, with fluorescent substrates Bombyx mori
3.2.1.14 37
-
assay at, with chitin substrates Bombyx mori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 4
-
exochitinase mutant D306N Bombyx mori
3.2.1.14 6.2
-
wild-type exochitinase Bombyx mori