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Literature summary extracted from
- The role of carboxyl, guanidine and imidazole groups in catalysis by a midgut trehalase purified from an insect larvae (2004), Insect Biochem. Mol. Biol., 34, 1089-1099.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.2.1.28 | Mn2+ stabilizes the enzyme during purification | Spodoptera frugiperda |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.28 | amygdalin | competitive inhibition | Spodoptera frugiperda |  |
| 3.2.1.28 | mandelonitrile | competitive inhibition | Spodoptera frugiperda | |
| 3.2.1.28 | methyl-alpha-glucoside | weak competitive inhibition | Spodoptera frugiperda | |
| 3.2.1.28 | methyl-alpha-mannoside | weak competitive inhibition | Spodoptera frugiperda | |
| 3.2.1.28 | additional information | no inhibition by 1,10-phenanthroline at 2 mM, and by gentiobiose | Spodoptera frugiperda | |
| 3.2.1.28 | phlorizin | hyperbolic uncompetitive, binds only to enzyme-substrate complex | Spodoptera frugiperda | |
| 3.2.1.28 | prunasin | competitive inhibition | Spodoptera frugiperda | |
| 3.2.1.28 | Salicin | competitive inhibition | Spodoptera frugiperda | |
| 3.2.1.28 | Tris | competitive | Spodoptera frugiperda | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.28 | additional information | - |
additional information | kinetics | Spodoptera frugiperda | |
| 3.2.1.28 | 0.47 | - |
alpha,alpha-trehalose | pH 6.0, 30°C | Spodoptera frugiperda | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.28 | membrane | about 10% | Spodoptera frugiperda | 16020 | - |
| 3.2.1.28 | soluble | about 90% | Spodoptera frugiperda | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.28 | Mn2+ | activates, required | Spodoptera frugiperda | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 67000 | - |
x * 67000, SDS-PAGE | Spodoptera frugiperda |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.28 | alpha,alpha-trehalose + H2O | Spodoptera frugiperda | - |
2 D-glucose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.28 | Spodoptera frugiperda | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.28 | 2896fold from midgut of larvae, by ammonium sulfate precipitation, 2 steps of hydrophobic interaction and ion exchange chromatography | Spodoptera frugiperda |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.2.1.28 | alpha,alpha-trehalose + H2O = beta-D-glucose + alpha-D-glucose | role of carboxyl, guanidine and imidazole groups in catalysis | Spodoptera frugiperda |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.28 | larva | - |
Spodoptera frugiperda | - |
| 3.2.1.28 | midgut | larval | Spodoptera frugiperda | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 168 | - |
purified enzyme | Spodoptera frugiperda |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.28 | alpha,alpha-trehalose + H2O | - |
Spodoptera frugiperda | 2 D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.28 | ? | x * 67000, SDS-PAGE | Spodoptera frugiperda |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.28 | trehalase | - |
Spodoptera frugiperda |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 30 | - |
assay at | Spodoptera frugiperda |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 7 | - |
- |
Spodoptera frugiperda |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 4 | 8.5 | 90% of maximal activity at pH 6.0, maximal activity at pH 7.0 | Spodoptera frugiperda |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.28 | additional information | - |
additional information | inhibition kinetics | Spodoptera frugiperda | |
| 3.2.1.28 | 0.09 | - |
phlorizin | pH 6.0, 30°C | Spodoptera frugiperda | |
| 3.2.1.28 | 0.21 | - |
amygdalin | pH 6.0, 30°C | Spodoptera frugiperda | |
| 3.2.1.28 | 0.55 | - |
Tris | pH 6.0, 30°C | Spodoptera frugiperda | |
| 3.2.1.28 | 0.92 | - |
prunasin | pH 6.0, 30°C | Spodoptera frugiperda | |
| 3.2.1.28 | 1.14 | - |
mandelonitrile | pH 6.0, 30°C | Spodoptera frugiperda | |
| 3.2.1.28 | 6.2 | - |
methyl-alpha-mannoside | pH 6.0, 30°C | Spodoptera frugiperda | |
| 3.2.1.28 | 19 | - |
Salicin | pH 6.0, 30°C | Spodoptera frugiperda | |
| 3.2.1.28 | 89 | - |
methyl-alpha-glucoside | pH 6.0, 30°C | Spodoptera frugiperda | |
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