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Literature summary extracted from

  • Bertin, P.B.; Lozzi, S.P.; Howell, J.K.; Restrepo-Cadavid, G.; Neves, D.; Teixeira, A.R.L.; De Sousa, M.V.; Norris, S.J.; Santana, J.M.
    The thermophilic, homohexameric aminopeptidase of Borrelia burgdorferi is a member of the M29 family of metallopeptidases (2005), Infect. Immun., 73, 2253-2261.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.4.11.10 pharmacology the enzyme ia a target for development of drugs in therapy of Lyme disease caused by Borrelia burgdorferi Borreliella burgdorferi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.10 1,10-phenanthroline complete inhibition at 0.25 mM, reversible by Zn2+, not by other divalent cations, overview Borreliella burgdorferi
3.4.11.10 bestatin complete inhibition at 0.1 mM, not reversible by divalent cations Borreliella burgdorferi
3.4.11.10 EDTA 50% inhibition at 10 mM, reversible by divalent cations, best by Zn2+ Borreliella burgdorferi
3.4.11.10 additional information no or poor inhibition by PMSF, E64, TLCK, pepstatin A, and leupeptin Borreliella burgdorferi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.10 Zn2+ dependent on Borreliella burgdorferi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.10 50000
-
 6 * 50000, SDS-PAGE Borreliella burgdorferi
3.4.11.10 300000
-
 gel filtration Borreliella burgdorferi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.11.10 additional information Borreliella burgdorferi the enzyme is probably involved in amino acid supply in the organism deficient in amino acid synthesis pathways, and/or in peptide and/or protein processing ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.10 Borreliella burgdorferi O51096 gene BB0069
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.10 native enzyme 32fold to homogeneity by anion exchange chromatography and gel filtration Borreliella burgdorferi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.10 0.001
-
 purified enzyme Borreliella burgdorferi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.10 L-Leu-7-amido-4-methylcoumarin + H2O
-
 Borreliella burgdorferi L-Leu + 7-amino-4-methylcoumarin
-
 ?
3.4.11.10 additional information the enzyme is probably involved in amino acid supply in the organism deficient in amino acid synthesis pathways, and/or in peptide and/or protein processing Borreliella burgdorferi ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.10 hexamer 6 * 50000, SDS-PAGE Borreliella burgdorferi
3.4.11.10 More oligomerization is required for activity, the monomer is inactive, the enzyme contains no disulfide bonds, peptide mass fingerprinting, overview Borreliella burgdorferi

Synonyms

EC Number Synonyms Comment Organism
3.4.11.10 aminopeptidase II
-
 Borreliella burgdorferi
3.4.11.10 More the enzyme is a member of the M29 family of metallopeptidases Borreliella burgdorferi
3.4.11.10 TAP
-
 Borreliella burgdorferi
3.4.11.10 thermophilic aminopeptidase
-
 Borreliella burgdorferi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.10 60
-
-
 Borreliella burgdorferi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.10 additional information
-
 thermal inactivation through transition of the hexameric to the monomeric form Borreliella burgdorferi
3.4.11.10 25 50 purified enzyme, completely stable for 240 min Borreliella burgdorferi
3.4.11.10 60
-
 purified enzyme, no loss activity within 10 min, 240 min: loss of 12.5% activity Borreliella burgdorferi
3.4.11.10 70
-
 purified enzyme, loss of 75% activity within 10 min, 240 min: loss of 91% activity Borreliella burgdorferi
3.4.11.10 100
-
 purified enzyme, 10 min, complete inactivation Borreliella burgdorferi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.10 7.5
-
-
 Borreliella burgdorferi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.10 6 9 20% of maximal activity at pH 6.0, 50% at pH 9.0 Borreliella burgdorferi