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Literature summary extracted from

  • Kawamoto, S.; Shibano, Y.; Fukushima, J.; Ishii, N.; Morihara, K.; Okuda, K.
    Site-directed mutagenesis of Glu-141 and His-223 in Pseudomonas aeruginosa elastase: catalytic activity, processing, and protective activity of the elastase against Pseudomonas infection (1993), Infect. Immun., 61, 1400-1405.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.4.24.26 medicine the protective activity of elastase mutants against Pseudomonas infections may be useful in producing vaccines Pseudomonas aeruginosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.26 expression of mutants E141D, E141Q, E141G, H223G, H223E, and H223L in Escherichia coli JM109 Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.26 E141D no activity Pseudomonas aeruginosa
3.4.24.26 E141G no activity Pseudomonas aeruginosa
3.4.24.26 E141Q no activity Pseudomonas aeruginosa
3.4.24.26 H223E no activity Pseudomonas aeruginosa
3.4.24.26 H223G no activity Pseudomonas aeruginosa
3.4.24.26 H223L no activity Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.26 membrane mutants E141D, E141Q, E141G, H223G, H223E, and H223L Pseudomonas aeruginosa 16020
-
3.4.24.26 periplasm wild-type Pseudomonas aeruginosa
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.26 33000
-
 x * 33000, SDS-PAGE, mature elastase Pseudomonas aeruginosa
3.4.24.26 51000
-
 x * 51000, SDS-PAGE, precursor form of elastase Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.26 Pseudomonas aeruginosa P14756 IFO 3455
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.26
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.26 azocasein + H2O 37°C, pH 8.0 Pseudomonas aeruginosa ?
-
 ?
3.4.24.26 elastin Congo red + H2O pH 8.0 Pseudomonas aeruginosa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.26 ? x * 33000, SDS-PAGE, mature elastase Pseudomonas aeruginosa
3.4.24.26 ? x * 51000, SDS-PAGE, precursor form of elastase Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
3.4.24.26 Pseudomonas aeruginosa elastase
-
 Pseudomonas aeruginosa