Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kessler, E.; Israel, M.; Landshman, N.; Chechick, A.; Blumberg, S.
    In vitro inhibition of Pseudomonas aeruginosa elastase by metal-chelating peptide derivatives (1982), Infect. Immun., 38, 716-723.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.4.24.26 medicine 2-mercaptoacetyl-L-phenylalanyl-L-leucine represents a class of potent elastase inhibitors that might prove useful in the management of Pseudomonas aeruginosa infections Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.26 2-mercaptoacetyl-Leu-Dphe
-
 Pseudomonas aeruginosa
3.4.24.26 2-mercaptoacetyl-Leu-Phe
-
 Pseudomonas aeruginosa
3.4.24.26 2-mercaptoacetyl-Phe-Leu at 0.1 mM 96% inhibition with azocasein as substrate, 97% inhibition with elastin as substrate and 97% inhibition with cartilage as substrate, at 0.01 mM 77% inhibition with azocasein as substrate, 33% inhibition with elastin as substrate and 66% inhibition with cartilage as substrate Pseudomonas aeruginosa
3.4.24.26 benzyloxycarbonyl-Gly-NHOH at 14 mM 98% inhibition with azocasein as substrate, 95% inhibition with elastin as substrate and 95% inhibition with cartilage as substrate, at 1.4 mM 77% inhibition with azocasein as substrate, 84% inhibition with elastin as substrate and 67% inhibition with cartilage as substrate Pseudomonas aeruginosa
3.4.24.26 benzyloxycarbonyl-L-leucine
-
 Pseudomonas aeruginosa
3.4.24.26 benzyloxycarbonyl-L-phenylalanine
-
 Pseudomonas aeruginosa
3.4.24.26 benzyloxycarbonyl-Leu-NHOH at 5.0 mM 98% inhibition with azocasein as substrate, 100% inhibition with elastin as substrate and 94% inhibition with cartilage as substrate, at 0.5 mM 76% inhibition with azocasein as substrate, 93% inhibition with elastin as substrate and 57% inhibition with cartilage as substrate Pseudomonas aeruginosa
3.4.24.26 benzyloxycarbonyl-Phe-NHOH
-
 Pseudomonas aeruginosa
3.4.24.26 Leu-Phe
-
 Pseudomonas aeruginosa
3.4.24.26 Phe-Leu
-
 Pseudomonas aeruginosa
3.4.24.26 phosphoryl-L-leucyl-L-phenylalanine at 0.1 mM 98% inhibition with azocasein as substrate, 94% inhibition with elastin as substrate and 98% inhibition with cartilage as substrate, at 0.01 mM 83% inhibition with azocasein as substrate, 87% inhibition with elastin as substrate and 89% inhibition with cartilage as substrate Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.26 35000
-
 x * 35000, SDS-PAGE Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.26 Pseudomonas aeruginosa P14756 Habs serotype 1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.26
-
 Pseudomonas aeruginosa

Storage Stability

EC Number Storage Stability Organism
3.4.24.26 -20°C, 0.02 M Tris-hydrochloride, 0.5 mM CaCl2 (pH 7.5), 1 year, less than 10% loss of activity Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.26 azocasein + H2O pH 7.5, 37°C Pseudomonas aeruginosa ?
-
 ?
3.4.24.26 cartilage + H2O major components proteoglycans and collagen Pseudomonas aeruginosa ?
-
 ?
3.4.24.26 Elastin + H2O pH 7.5, 37°C Pseudomonas aeruginosa ?
-
 ?
3.4.24.26 furylacryloyl-glycyl-L-leucyl-L-alanine + H2O pH 7.5, 25°C Pseudomonas aeruginosa furylacryloyl-glycine + L-leucyl-L-alanine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.26 ? x * 35000, SDS-PAGE Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
3.4.24.26 Pseudomonas aeruginosa elastase
-
 Pseudomonas aeruginosa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.24.26 0.0002
-
 phosphoryl-L-leucyl-L-phenylalanine at pH 7.5 and 25°C, with furylacryloyl-glycyl-L-leucyl-L-alanine as substrate Pseudomonas aeruginosa
3.4.24.26 0.0002
-
 2-mercaptoacetyl-Phe-Leu at pH 7.5 and 25°C, with furylacryloyl-glycyl-L-leucyl-L-alanine as substrate Pseudomonas aeruginosa
3.4.24.26 0.0015
-
 2-mercaptoacetyl-Leu-Phe at pH 7.5 and 25°C, with furylacryloyl-glycyl-L-leucyl-L-alanine as substrate Pseudomonas aeruginosa
3.4.24.26 0.011
-
 benzyloxycarbonyl-Leu-NHOH at pH 7.5 and 25°C, with furylacryloyl-glycyl-L-leucyl-L-alanine as substrate Pseudomonas aeruginosa
3.4.24.26 0.021
-
 benzyloxycarbonyl-Phe-NHOH at pH 7.5 and 25°C, with furylacryloyl-glycyl-L-leucyl-L-alanine as substrate Pseudomonas aeruginosa
3.4.24.26 0.028
-
 benzyloxycarbonyl-Gly-NHOH at pH 7.5 and 25°C, with furylacryloyl-glycyl-L-leucyl-L-alanine as substrate Pseudomonas aeruginosa
3.4.24.26 0.034
-
 2-mercaptoacetyl-Leu-Dphe at pH 7.5 and 25°C, with furylacryloyl-glycyl-L-leucyl-L-alanine as substrate Pseudomonas aeruginosa
3.4.24.26 0.4
-
 Phe-Leu at pH 7.5 and 25°C, with furylacryloyl-glycyl-L-leucyl-L-alanine as substrate Pseudomonas aeruginosa
3.4.24.26 1.1
-
 Leu-Phe at pH 7.5 and 25°C, with furylacryloyl-glycyl-L-leucyl-L-alanine as substrate Pseudomonas aeruginosa
3.4.24.26 5
-
 benzyloxycarbonyl-L-phenylalanine at pH 7.5 and 25°C, with furylacryloyl-glycyl-L-leucyl-L-alanine as substrate Pseudomonas aeruginosa
3.4.24.26 6.2
-
 benzyloxycarbonyl-L-leucine at pH 7.5 and 25°C, with furylacryloyl-glycyl-L-leucyl-L-alanine as substrate Pseudomonas aeruginosa