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Literature summary extracted from

  • Butler, M.J.
    Aminopeptidase N (Streptomyces lividans) (2004), Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. ), 1, 322-323.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.2 gene pepN, DNA and amino acid sequence determination and analysis, overvexpression Streptomyces lividans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.2 1,10-phenanthroline
-
Streptomyces lividans
3.4.11.2 EDTA
-
Streptomyces lividans
3.4.11.2 additional information no inhibition by PMSF Streptomyces lividans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.2 intracellular the enzyme contains no secretion signal sequence Streptomyces lividans 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.2 Zn2+ metallopeptidase, zinc-binding motif HELAH Streptomyces lividans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.2 95000
-
gel filtration Streptomyces lividans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.11.2 additional information Streptomyces lividans the enzyme might be involved in the turnover of intracellular peptides ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.2 Streptomyces lividans
-
gene pepN
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.2 by ammonium sulfate fractionation, ion exchange chromatography and gel filtration Streptomyces lividans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.2 Gly-4-nitroanilide + H2O low activity Streptomyces lividans Gly + 4-nitroaniline
-
?
3.4.11.2 L-Ala-4-nitroanilide + H2O
-
Streptomyces lividans L-Ala + 4-nitroaniline
-
?
3.4.11.2 L-Arg-2-naphthylamide + H2O
-
Streptomyces lividans L-Arg + 2-naphthylamine
-
?
3.4.11.2 L-Arg-4-nitroanilide + H2O
-
Streptomyces lividans L-Arg + 4-nitroaniline
-
?
3.4.11.2 L-Leu-2-naphthylamide + H2O
-
Streptomyces lividans L-Leu + 2-naphthylamine
-
?
3.4.11.2 L-Leu-4-nitroanilide + H2O best substrate Streptomyces lividans L-Leu + 4-nitroaniline
-
?
3.4.11.2 L-Lys-4-nitroanilide + H2O
-
Streptomyces lividans L-Lys + 4-nitroaniline
-
?
3.4.11.2 L-Met-4-nitroanilide + H2O
-
Streptomyces lividans L-Met + 4-nitroaniline
-
?
3.4.11.2 L-Phe-4-nitroanilide + H2O slow activity Streptomyces lividans L-Phe + 4-nitroaniline
-
?
3.4.11.2 L-Pro-4-nitroanilide + H2O low activity Streptomyces lividans L-Pro + 4-nitroaniline
-
?
3.4.11.2 L-Ser-4-nitroanilide + H2O slow activity Streptomyces lividans L-Ser + 4-nitroaniline
-
?
3.4.11.2 additional information the enzyme might be involved in the turnover of intracellular peptides Streptomyces lividans ?
-
?
3.4.11.2 additional information no activity with L-Val-4-nitroanilide, L-Glu-4-nitroanilide, and L-Asp-4-nitroanilide, no activity with N-alpha-benzoyl-L-Arg-2-naphthylamide Streptomyces lividans ?
-
?

Subunits

EC Number Subunits Comment Organism
3.4.11.2 monomer 1 * 95000, SDS-PAGE Streptomyces lividans

Synonyms

EC Number Synonyms Comment Organism
3.4.11.2 aminopeptidase N
-
Streptomyces lividans
3.4.11.2 More the enzyme belongs to the peptidase family M1 Streptomyces lividans
3.4.11.2 PepN
-
Streptomyces lividans