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Literature summary extracted from

  • Foulon, T.; Cadel, S.; Piesse, C.; Cohen, P.
    Aminopeptidase B (2004), Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds) Academic Press, 1, 328-332.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.11.6 additional information the enzyme is up-regulated during long-term activation of normal and lymphoma T-cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.6 1,10-phenanthroline reversible by Zn2+ Rattus norvegicus
3.4.11.6 4-chloromercuribenzoate
-
 Rattus norvegicus
3.4.11.6 amastatin
-
 Rattus norvegicus
3.4.11.6 arphamenine A
-
 Rattus norvegicus
3.4.11.6 Arphamenine B
-
 Rattus norvegicus
3.4.11.6 bestatin
-
 Rattus norvegicus
3.4.11.6 DTT
-
 Rattus norvegicus
3.4.11.6 EDTA
-
 Rattus norvegicus
3.4.11.6 EGTA
-
 Rattus norvegicus
3.4.11.6 additional information no inhibition by PMSF, pepstatin, and aprotinin Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.6 0.005
-
 Arg-Lys-somatostatin-14
-
 Rattus norvegicus
3.4.11.6 0.014
-
 Arg-alpha-atrial natriuretic factor1-20
-
 Rattus norvegicus
3.4.11.6 0.02
-
 L-Arg-2-naphthylamide
-
 Rattus norvegicus
3.4.11.6 0.036
-
 L-Lys-2-naphthylamide
-
 Rattus norvegicus
3.4.11.6 0.058
-
 Arg-neurokinin A
-
 Rattus norvegicus
3.4.11.6 0.111
-
 Arg-Leu-enkephalin
-
 Rattus norvegicus
3.4.11.6 0.125
-
 Arg-Met-enkephalin
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.6 acrosome of germinal cells, proacrosmic granule of round spermatids Rattus norvegicus
-
-
3.4.11.6 cytoplasm of late spermatids Rattus norvegicus 5737
-
3.4.11.6 extracellular the enzyme is secreted, in PC-12 cells the active enzyme is associated to the external surface of the plassma mamabrane Rattus norvegicus
-
-
3.4.11.6 Golgi apparatus of germinal cells Rattus norvegicus 5794
-
3.4.11.6 secondary lysosome of late spermatids Rattus norvegicus 5767
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.6 Cl- activates at physiological concentrations Mus musculus
3.4.11.6 Cl- activates at physiological concentrations Homo sapiens
3.4.11.6 Cl- activates at physiological concentrations around 150 mM, dependent on the target peptide Rattus norvegicus
3.4.11.6 Zn2+ zinc-metallopeptidase Mus musculus
3.4.11.6 Zn2+ zinc-metallopeptidase Homo sapiens
3.4.11.6 Zn2+ dependent on, zinc-metallopeptidase, the enzyme contains the binding motif HEXXHX18E Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.6 72300
-
 1 * 95500, liver enzyme, 1 * 72300, testis enzyme Rattus norvegicus
3.4.11.6 95500
-
 1 * 95500, liver enzyme, 1 * 72300, testis enzyme Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.11.6 additional information Rattus norvegicus the enzyme probably is involved in the final stages of peptide and protein precursor processing and maturation mechanisms via the exopeptidase pathway and thereby in some inflammatory processes and tumour development, the enzyme from testis preferably removes Arg and/or Lys residues from the N-terminus of various peptides ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.6 Homo sapiens
-
-
-
3.4.11.6 Mus musculus
-
-
-
3.4.11.6 Rattus norvegicus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.11.6 release of N-terminal Arg and Lys from oligopeptides when P1' is not Pro. Also acts on arylamides of Arg and Lys exopeptidase, the enzyme is strictly specific for the removal of N-terminal basic residues from peptides and proteins, no activity with substrate possessing Pro at the P1 position Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.6 BE(2)-M17 cell neuroblastoma cell line Homo sapiens
-
3.4.11.6 epididymis
-
 Rattus norvegicus
-
3.4.11.6 GH3 cell pituitary tumor-derived cell line Rattus norvegicus
-
3.4.11.6 heart
-
 Rattus norvegicus
-
3.4.11.6 JURKAT cell T-lymphoma cell line Homo sapiens
-
3.4.11.6 K-562 cell chronic myeloid leukemia cell line Homo sapiens
-
3.4.11.6 kidney
-
 Rattus norvegicus
-
3.4.11.6 large intestine
-
 Rattus norvegicus
-
3.4.11.6 Leydig cell
-
 Rattus norvegicus
-
3.4.11.6 liver
-
 Rattus norvegicus
-
3.4.11.6 lung
-
 Rattus norvegicus
-
3.4.11.6 MOLT-4 cell T-cell line Homo sapiens
-
3.4.11.6 additional information the enzyme is up-regulated during long-term activation of normal and lymphoma T-cells Homo sapiens
-
3.4.11.6 Mrc5 cell lung cell line Homo sapiens
-
3.4.11.6 muscle
-
 Rattus norvegicus
-
3.4.11.6 pancreas
-
 Rattus norvegicus
-
3.4.11.6 PC-12 cell adrenal pheochromocytoma cell line Rattus norvegicus
-
3.4.11.6 pituitary gland
-
 Rattus norvegicus
-
3.4.11.6 pituitary gland cell line primary pituitary gland cell line Rattus norvegicus
-
3.4.11.6 small intestine
-
 Rattus norvegicus
-
3.4.11.6 spermatozoon
-
 Rattus norvegicus
-
3.4.11.6 spleen
-
 Rattus norvegicus
-
3.4.11.6 T-lymphocyte
-
 Homo sapiens
-
3.4.11.6 testis highest enzyme activity, mainly expressed in late spermatids during maturation Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.6 Arg-alpha-atrial natriuretic factor1-20 + H2O
-
 Rattus norvegicus Arg + alpha-atrial natriuretic factor1-20
-
 ?
3.4.11.6 Arg-Leu-enkephalin + H2O
-
 Rattus norvegicus Arg + Leu-enkephalin
-
 ?
3.4.11.6 Arg-Lys-somatostatin-14 + H2O
-
 Rattus norvegicus Arg-Lys + somatostatin-14
-
 ?
3.4.11.6 Arg-Met-enkephalin + H2O
-
 Rattus norvegicus Arg + Met-enkephalin
-
 ?
3.4.11.6 Arg-neurokinin A + H2O
-
 Rattus norvegicus Arg + neurokinin A
-
 ?
3.4.11.6 kallidin-10 + H2O
-
 Rattus norvegicus Lys + bradykinin
-
 ?
3.4.11.6 L-Arg-2-naphthylamide + H2O
-
 Rattus norvegicus L-Arg + 2-naphthylamine
-
 ?
3.4.11.6 L-Lys-2-naphthylamide + H2O
-
 Rattus norvegicus L-Lys + 2-naphthylamine
-
 ?
3.4.11.6 leukotriene A4 + H2O weak in vitro activity Rattus norvegicus L-Arg + leukotriene B4
-
 ?
3.4.11.6 additional information the enzyme probably is involved in the final stages of peptide and protein precursor processing and maturation mechanisms via the exopeptidase pathway and thereby in some inflammatory processes and tumour development, the enzyme from testis preferably removes Arg and/or Lys residues from the N-terminus of various peptides Rattus norvegicus ?
-
 ?
3.4.11.6 additional information the enzyme is strictly specific for the removal of N-terminal basic residues from peptides and proteins Mus musculus ?
-
 ?
3.4.11.6 additional information the enzyme is strictly specific for the removal of N-terminal basic residues from peptides and proteins Homo sapiens ?
-
 ?
3.4.11.6 additional information the enzyme is strictly specific for the removal of N-terminal basic residues from peptides and proteins, no activity with bradykinin, no activity with substrate possessing Pro at the P1 position Rattus norvegicus ?
-
 ?
3.4.11.6 thymopentin + H2O
-
 Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.6 monomer 1 * 95500, liver enzyme, 1 * 72300, testis enzyme Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.4.11.6 Ap-B
-
 Rattus norvegicus
3.4.11.6 arginyl aminopeptidase
-
 Rattus norvegicus
3.4.11.6 arylamidase
-
 Rattus norvegicus
3.4.11.6 Cl--activated arinine aminopeptidase
-
 Rattus norvegicus
3.4.11.6 cytosol aminopeptidase IV
-
 Rattus norvegicus
3.4.11.6 More the enzyme belongs to the M1 peptidase family Mus musculus
3.4.11.6 More the enzyme belongs to the M1 peptidase family Homo sapiens
3.4.11.6 More the enzyme belongs to the M1 peptidase family, and is distinct from the leukotriene A4 hydrolase, bleomycin hydrolase, and puromycin-sensitive aminopeptidase Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.6 7
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.6 additional information
-
 broad activity range Rattus norvegicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.11.6 Rattus norvegicus experimental
-
 4.9
3.4.11.6 Rattus norvegicus theoretical
-
 5.83