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Literature summary extracted from

  • Motoshima, H.; Kaminogawa, S.
    Bacillus aminopeptidase I (2004), Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds), 1, 968-970.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.11.22 tert-butanol 20% activation at 10% at 20-90°C Geobacillus stearothermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.22 EDTA 10 mM, no inhibition at pH 8.1, but conversion into the apoenzyme at pH 6.0 Geobacillus stearothermophilus
3.4.11.22 Urea at 8 M, inhibition of 7% of the enzyme activity with Gly-Leu-Tyr, but 75% of the activity with L-Leu-4-nitroanilide Geobacillus stearothermophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.22 membrane the enzyme exists in a membrane and a soluble variant with slightly differing substrate specificities and thermostabilities Geobacillus stearothermophilus 16020
-
3.4.11.22 soluble the enzyme exists in a membrane and a soluble variant with slightly differing substrate specificities and thermostabilities Geobacillus stearothermophilus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.22 Co2+ binding results in much higher activity compared to Zn2+, Co2+ or Zn2+ is required for maximal activity, metallopeptidase Geobacillus stearothermophilus
3.4.11.22 Zn2+ binding results in much lower activity compared to Co2+, very tight binding, Co2+ or Zn2+ is required for maximal activity, metallopeptidase Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.22 36500
-
 12 * 36500, heterododecamer with two types of subunits with similar MW, the enzyme shows different hybrid types, overview, the enzymatic activity of the alpha-subunit differs from that of the beta-subunit Geobacillus stearothermophilus
3.4.11.22 400000
-
 sedimentation equilibrium centrifugation analysis Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.22 Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.22 native enzyme 19fold Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.22 Gly-Leu-Tyr + H2O
-
 Geobacillus stearothermophilus Gly + Leu-Tyr
-
 ?
3.4.11.22 L-Leu-4-nitroanilide + H2O substrate of the alpha-subunit, but not of the beta-subunit Geobacillus stearothermophilus L-Leu + 4-nitroaniline
-
 ?
3.4.11.22 additional information the alpha-subunits hydrolyzes peptides with neutral N-terminal amino acids while the beta-subunit acts on peptides with acidic N-terminal Glu or Asp residues Geobacillus stearothermophilus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.22 dodecamer 12 * 36500, heterododecamer with two types of subunits with similar MW, the enzyme shows different hybrid types, overview, the enzymatic activity of the alpha-subunit differs from that of the beta-subunit Geobacillus stearothermophilus

Synonyms

EC Number Synonyms Comment Organism
3.4.11.22 API
-
 Geobacillus stearothermophilus
3.4.11.22 Bacillus aminopeptidase I
-
 Geobacillus stearothermophilus
3.4.11.22 More the enzyme belongs to the peptidase family M42 Geobacillus stearothermophilus
3.4.11.22 thermophilic aminopeptidase
-
 Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.22 65
-
 with substrate Gly-Leu-Tyr Geobacillus stearothermophilus
3.4.11.22 90
-
 with substrate L-Leu-4-nitroanilide Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.22 additional information
-
 the apo- and cobalt-free enzyme is thermolabile Geobacillus stearothermophilus
3.4.11.22 80
-
 loss of 20% activity within 30 min, stable for several hours Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.22 7.5 8 with substrate L-Leu-4-nitroanilide Geobacillus stearothermophilus
3.4.11.22 9.2 9.5 with substrate Gly-Leu-Tyr Geobacillus stearothermophilus