EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.22.B20 | expression in Escherichia coli | Pyrococcus furiosus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.22.B20 | DFP | - |
Pyrococcus furiosus | |
3.4.22.B20 | PMSF | - |
Pyrococcus furiosus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.22.B20 | 18800 | - |
monomer, calculated from amino acid sequence | Pyrococcus furiosus |
3.4.22.B20 | 59000 | - |
trimer, gel filtration | Pyrococcus furiosus |
3.4.22.B20 | 66000 | - |
after heating at 95°C in 1% SDS for 24 h, SDS-PAGE | Pyrococcus furiosus |
3.4.22.B20 | 86000 | - |
SDS-PAGE, the protein is not fully denatured by SDS | Pyrococcus furiosus |
3.4.22.B20 | 124000 | - |
hexamer, gel filtration | Pyrococcus furiosus |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.4.22.B20 | 2-mercaptoethanol | the protease retains some proteolytic activity in the presence of 2-mercaptoethanol | Pyrococcus furiosus |
3.4.22.B20 | dithiothreitol | the protease retains some proteolytic activity in the presence of dithiothreitol | Pyrococcus furiosus |
3.4.22.B20 | SDS | the protease is resistant to SDS | Pyrococcus furiosus |
3.4.22.B20 | urea | the protease is resistant to urea | Pyrococcus furiosus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.22.B20 | Pyrococcus furiosus | Q51732 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.22.B20 | - |
Pyrococcus furiosus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.B20 | azocasein + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
3.4.22.B20 | Gelatin + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
3.4.22.B20 | Suc-Ala-Ala-Phe-NHMec + H2O | pH 7, NHMec = 7-amino-4-methylcoumaryl | Pyrococcus furiosus | Suc-Ala-Ala-Phe + 7-amino-4-methylcoumarin | NHMec = 7-amino-4-methylcoumaryl | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.22.B20 | hexamer | 6 * 18800, gel filtration | Pyrococcus furiosus |
3.4.22.B20 | trimer | 3 * 18800, gel filtration | Pyrococcus furiosus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.22.B20 | PfpI endopeptidase | - |
Pyrococcus furiosus |
3.4.22.B20 | PfpI protease | - |
Pyrococcus furiosus |
3.4.22.B20 | Pyrococcus furiosus protease I | - |
Pyrococcus furiosus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.22.B20 | 86 | - |
- |
Pyrococcus furiosus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.22.B20 | 98 | - |
the enzyme has a 33 h half-life at 98°C after heating in 1% SDS | Pyrococcus furiosus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.4.22.B20 | Pyrococcus furiosus | trimer, experimentally determined | 3.9 | 3.7 |
3.4.22.B20 | Pyrococcus furiosus | 18.8 kDa subunit, calculated from amino acid sequence | - |
6.1 |
3.4.22.B20 | Pyrococcus furiosus | hexamer, experimentally determined | - |
6.1 |