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Literature summary extracted from

  • Hicks, P.M.; Chang, L.S.; Kelly, R.M.
    Pyrococcus furiosus protease I (2004), Handbook of Proteolytic Enzymes (Barrett, A. J. ; Rawlings, N. D. ; Woessner, eds. ), 2, 1393-1395.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.B20 expression in Escherichia coli Pyrococcus furiosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.B20 DFP
-
Pyrococcus furiosus
3.4.22.B20 PMSF
-
Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.B20 18800
-
monomer, calculated from amino acid sequence Pyrococcus furiosus
3.4.22.B20 59000
-
trimer, gel filtration Pyrococcus furiosus
3.4.22.B20 66000
-
after heating at 95°C in 1% SDS for 24 h, SDS-PAGE Pyrococcus furiosus
3.4.22.B20 86000
-
SDS-PAGE, the protein is not fully denatured by SDS Pyrococcus furiosus
3.4.22.B20 124000
-
hexamer, gel filtration Pyrococcus furiosus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.4.22.B20 2-mercaptoethanol the protease retains some proteolytic activity in the presence of 2-mercaptoethanol Pyrococcus furiosus
3.4.22.B20 dithiothreitol the protease retains some proteolytic activity in the presence of dithiothreitol Pyrococcus furiosus
3.4.22.B20 SDS the protease is resistant to SDS Pyrococcus furiosus
3.4.22.B20 urea the protease is resistant to urea Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.B20 Pyrococcus furiosus Q51732
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.B20
-
Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.B20 azocasein + H2O
-
Pyrococcus furiosus ?
-
?
3.4.22.B20 Gelatin + H2O
-
Pyrococcus furiosus ?
-
?
3.4.22.B20 Suc-Ala-Ala-Phe-NHMec + H2O pH 7, NHMec = 7-amino-4-methylcoumaryl Pyrococcus furiosus Suc-Ala-Ala-Phe + 7-amino-4-methylcoumarin NHMec = 7-amino-4-methylcoumaryl ?

Subunits

EC Number Subunits Comment Organism
3.4.22.B20 hexamer 6 * 18800, gel filtration Pyrococcus furiosus
3.4.22.B20 trimer 3 * 18800, gel filtration Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
3.4.22.B20 PfpI endopeptidase
-
Pyrococcus furiosus
3.4.22.B20 PfpI protease
-
Pyrococcus furiosus
3.4.22.B20 Pyrococcus furiosus protease I
-
Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.B20 86
-
-
Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.B20 98
-
the enzyme has a 33 h half-life at 98°C after heating in 1% SDS Pyrococcus furiosus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.22.B20 Pyrococcus furiosus trimer, experimentally determined 3.9 3.7
3.4.22.B20 Pyrococcus furiosus 18.8 kDa subunit, calculated from amino acid sequence
-
6.1
3.4.22.B20 Pyrococcus furiosus hexamer, experimentally determined
-
6.1